Postgraduate Program in Veterinary Sciences, School of Veterinary, State University of Ceará. Dr. Silas Munguba, 1700, Campus do Itaperi, CEP: 60714-903, Fortaleza, Ceará, Brazil.
Specialized Medical Mycology Center, Postgraduate Program in Medical Microbiology, Department of Pathology and Legal Medicine, Federal University of Ceará. Rua Coronel Nunes de Melo, 1315 - Rodolfo Teófilo - CEP: 60430-275, Fortaleza, Ceará, Brazil.
J Med Microbiol. 2024 Mar;73(3). doi: 10.1099/jmm.0.001815.
Cryptococcal biofilms have been associated with persistent infections and antifungal resistance. Therefore, strategies, such as the association of natural compounds and antifungal drugs, have been applied for the prevention of biofilm growth. Moreover, the pathogenicity model has been used to investigate the capacity to inhibit the pathogenicity of . Anthraquinones and antifungals are associated with preventing biofilm formation and disrupting these communities. Antraquinones reduced the pathogenicity in the model. This study aimed to evaluate the interaction between aloe emodin, barbaloin or chrysophanol and itraconazole or amphotericin B against growing and mature biofilms of . Compounds and antifungal drugs were added during biofilm formation or after 72 h of growth. Then, the metabolic activity was evaluated by the MTT reduction assay, the biomass by crystal-violet staining and the biofilm morphology by confocal laser scanning microscopy. pathogenicity was investigated using the nematode . Finally, pathogenicity inhibition by aloe emodin, barbarloin and chrysophanol was investigated using this model. Anthraquinone-antifungal combinations affected the development of biofilms with a reduction of over 60 % in metabolic activity and above 50 % in biomass. Aloe emodin and barbaloin increased the anti-biofilm activity of antifungal drugs. Chrysophanol potentiated the effect of itraconazole against biofilms. The mortality rate reached 76.7 % after the worms were exposed to for 96 h. Aloe emodin, barbaloin and chrysophanol reduced the pathogenicity with mortality rates of 61.12 %, 65 % and 53.34 %, respectively, after the worms were exposed for 96 h to and these compounds at same time. These results highlight the potential activity of anthraquinones to increase the effectiveness of antifungal drugs against cryptococcal biofilms.
隐球菌生物膜与持续性感染和抗真菌耐药性有关。因此，已经应用了一些策略，如天然化合物和抗真菌药物的联合应用，以预防生物膜的生长。此外，还使用了致病性模型来研究抑制致病性的能力。蒽醌类化合物和抗真菌药物与预防生物膜形成和破坏这些群落有关。蒽醌类化合物降低了模型中的致病性。本研究旨在评估芦荟大黄素、巴豆苷或大黄酚与伊曲康唑或两性霉素 B 之间的相互作用，以抑制生长和成熟的隐球菌生物膜。在生物膜形成过程中或生长 72 小时后加入化合物和抗真菌药物。然后，通过 MTT 还原测定评估代谢活性，通过结晶紫染色评估生物量，通过共聚焦激光扫描显微镜评估生物膜形态。使用线虫研究了致病性。最后，使用该模型研究了芦荟大黄素、巴豆苷和大黄酚对致病性的抑制作用。蒽醌-抗真菌联合作用影响生物膜的发育，代谢活性降低超过 60%，生物量降低超过 50%。芦荟大黄素和巴豆苷增加了抗真菌药物的抗生物膜活性。大黄酚增强了伊曲康唑对生物膜的作用。线虫暴露于 96 小时后，死亡率达到 76.7%。线虫暴露于 96 小时后，芦荟大黄素、巴豆苷和大黄酚的死亡率分别为 61.12%、65%和 53.34%，同时降低了隐球菌的致病性。这些结果突出了蒽醌类化合物增加抗真菌药物对隐球菌生物膜有效性的潜在活性。
