Department of Physics, Institute of Science, Banaras Hindu University, Varanasi, 221005, India.
Government Polytecnic Rajgrah, Mirzapur, Bathua, 231001, India.
J Fluoresc. 2024 May;34(3):1441-1451. doi: 10.1007/s10895-024-03665-1. Epub 2024 Mar 26.
Aspirin is a commonly used nonsteroidal anti-inflammatory drug, associated with many adverse effects. The adverse effects of aspirin such as tinnitus, Reye's syndrome and gastrointestinal bleeding are caused due to conversion of aspirin into its active metabolite salicylic acid after oral intake. Glutathione is a naturally occurring antioxidant produced by the liver and nerve cells in the central nervous system. It helps to metabolize toxins, break down free radicles, and support immune function. This study aims to investigate and explore the possibility of inhibiting aspirin to salicylic acid conversion in presence of glutathione at a molecular level using spectroscopic techniques such as UV-Visible absorption, time-Resolved and time-dependent fluorescence and theoretical DFT/ TD-DFT calculations. The results of steady state fluorescence spectroscopy and time-dependent fluorescence indicated that the aspirin to salicylic acid conversion is considerably inhibited in presence of glutathione. Further, the results presented here might have significant clinical implications for individuals with variations in glutathione level.
阿司匹林是一种常用的非甾体抗炎药，与许多不良反应有关。阿司匹林的不良反应，如耳鸣、瑞氏综合征和胃肠道出血，是由于口服后阿司匹林转化为其活性代谢物水杨酸引起的。谷胱甘肽是一种由肝脏和中枢神经系统神经细胞自然产生的抗氧化剂。它有助于代谢毒素、分解自由基，并支持免疫功能。本研究旨在使用光谱技术，如紫外-可见吸收、时间分辨和时间相关荧光以及理论 DFT/TD-DFT 计算，从分子水平上研究和探索在谷胱甘肽存在下抑制阿司匹林转化为水杨酸的可能性。稳态荧光光谱和时间相关荧光的结果表明，在谷胱甘肽存在下，阿司匹林转化为水杨酸的速度明显受到抑制。此外，这里呈现的结果可能对谷胱甘肽水平存在差异的个体具有重要的临床意义。
