Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Katowice, Poland.
Department of Plant Cytology and Embryology, Faculty of Biology, Jagiellonian University in Kraków, Kraków, Poland.
Methods Mol Biol. 2024;2791:45-56. doi: 10.1007/978-1-0716-3794-4_5.
Techniques based on the use of plant protoplasts are a convenient model for better understanding and observing developmental changes in the cells. The establishment of research tools based on protoplasts consists of many steps needed for optimization. Here, we describe the culture of morphogenic callus (MC)- and hypocotyl-derived protoplasts of common (Fagopyrum esculentum Moench) and Tartary (F. tataricum (L.) Gaertn.) buckwheat. Protoplasts embedding in agarose matrix and application of plant hormones, including phytosulfokine (PSK), enable the development of protoplast cultures and plant regeneration.
基于植物原生质体使用的技术是更好地理解和观察细胞发育变化的一种便捷模型。基于原生质体的研究工具的建立包括许多需要优化的步骤。在这里，我们描述了普通（荞麦 Fagopyrum esculentum Moench）和鞑靼（荞麦 F. tataricum (L.) Gaertn.）荞麦形态发生愈伤组织（MC）和下胚轴衍生原生质体的培养。将原生质体嵌入琼脂糖基质中和应用植物激素，包括植物磺基肽（PSK），可以促进原生质体培养和植物再生的发展。
