Jamshidi Zahra, Karami Manizheh, Khalili Mohsen, Roghani Mehrdad
Department of Biology, Shahed University, Tehran, Iran.
Department of Physiology, Shahed University, Tehran, Iran.
Indian J Endocrinol Metab. 2024 Jan-Feb;28(1):43-46. doi: 10.4103/ijem.ijem_364_22. Epub 2023 Jun 30.
Morphine induces ovarian cysts that cause obesity and disrupt sex hormone secretion. Baclofen, a gamma-aminobutyric acid receptor agonist, can help regulate sex hormones and reduce harmful blood lipids by protecting against morphine-induced gamma-aminobutyric acid inhibition. We investigated the prophylactic effect of baclofen in rats receiving morphine by comparing with the untreated groups.
Forty eight female Wistar rats were randomly divided into several groups, including control (saline 1 mL/kg, i.p.), morphine (5 mg/kg, i.p.), baclofen (10, 20, and 30 mg/kg, i.p.), and baclofen (10, 20, and 30 mg/kg) before morphine (5 mg/kg). Twenty four hours after the treatment, blood and serum samples were taken to check the levels of gonadotropins (LH & FSH) and lipid profile. The ovaries and uterus were also studied, and a proinflammatory nitric oxide (NO) diagnostic test was completed. The results were analyzed using analysis of variance (α = 0.05).
In comparison with the control group, the levels of LH and not FSH decreased in the morphine group and the number of ovarian cysts was more in the morphine group. These problems were not observed in the group of baclofen alone and baclofen + morphine. However, the triglyceride level increased slightly in the baclofen 30 mg/kg + morphine group. But the LDL level somewhat decreased. The proinflammatory NO system did not show significant activation in the ovary and uterus, except for the baclofen 10 mg/kg + baclofen group.
Morphine can cause ovarian cysts by lowering LH but baclofen prophylaxis can protect reproductive properties by adapting major metabolic changes.
吗啡会诱发卵巢囊肿，导致肥胖并扰乱性激素分泌。巴氯芬是一种γ-氨基丁酸受体激动剂，可通过防止吗啡诱导的γ-氨基丁酸抑制来帮助调节性激素并降低有害血脂。我们通过与未治疗组比较，研究了巴氯芬对接受吗啡的大鼠的预防作用。
48只雌性Wistar大鼠随机分为几组，包括对照组（腹腔注射1 mL/kg生理盐水）、吗啡组（腹腔注射5 mg/kg）、巴氯芬组（腹腔注射10、20和30 mg/kg）以及在注射吗啡（5 mg/kg）前注射巴氯芬（10、20和30 mg/kg）的组。治疗24小时后，采集血液和血清样本以检测促性腺激素（LH和FSH）水平和血脂情况。还对卵巢和子宫进行了研究，并完成了促炎一氧化氮（NO）诊断测试。结果采用方差分析进行分析（α = 0.05）。
与对照组相比，吗啡组LH水平下降而FSH水平未下降，且吗啡组卵巢囊肿数量更多。单独使用巴氯芬组和巴氯芬+吗啡组未观察到这些问题。然而，巴氯芬30 mg/kg +吗啡组甘油三酯水平略有升高，但低密度脂蛋白水平有所下降。除巴氯芬10 mg/kg +巴氯芬组外，促炎NO系统在卵巢和子宫中未显示出明显激活。
吗啡可通过降低LH导致卵巢囊肿，但巴氯芬预防可通过适应主要代谢变化来保护生殖特性。
