State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling, China.
Qingyang Longfeng Sponge City Construction Management and Operation Co., Ltd, Qingyang, China.
Microbiol Spectr. 2024 May 2;12(5):e0375623. doi: 10.1128/spectrum.03756-23. Epub 2024 Mar 27.
Zur (zinc uptake regulator) is a significant member of the Fur (ferric uptake regulator) superfamily, which is widely distributed in bacteria. Zur plays crucial roles in zinc homeostasis and influences cell development and environmental adaptation in various species. is a Gram-negative enteric that pathogen usually serves as a model organism in pathogenicity studies. The regulatory effects of Zur on the zinc transporter ZnuABC and the protein secretion system T6SS have been documented in . In this study, a comparative transcriptomics analysis between a ∆ mutant and the wild-type (WT) strain of was conducted using RNA-seq. This analysis revealed global regulation by Zur across multiple functional categories, including membrane transport, cell motility, and molecular and energy metabolism. Additionally, Zur mediates the homeostasis not only of zinc but also ferric and magnesium . There was a notable decrease in 35 flagellar biosynthesis and assembly-related genes, leading to reduced swimming motility in the ∆ mutant strain. Furthermore, Zur upregulated multiple simple sugar and oligopeptide transport system genes by directly binding to their promoters. The absence of Zur inhibited biofilm formation as well as reduced resistance to chloramphenicol and acidic stress. This study illustrates the comprehensive regulatory functions of Zur, emphasizing its importance in stress resistance and pathogenicity in .
Bacteria encounter diverse stresses in the environment and possess essential regulators to modulate the expression of genes in responding to the stresses for better fitness and survival. Zur (zinc uptake regulator) plays a vital role in zinc homeostasis. Studies of Zur from multiple species reviewed that it influences cell development, stress resistance, and virulence of bacteria. is an enteric pathogen that serves a model organism in the study of pathogenicity, virulence factors, and mechanism of environmental adaptation. In this study, transcriptomics analysis of Zur's regulons was conducted in . The functions of Zur as a global regulator in metal homeostasis, motility, nutrient acquisition, glycan metabolism, and nucleotide metabolism, in turn, increasing the biofilm formation, stress resistance, and virulence were reviewed. The importance of Zur in environmental adaptation and pathogenicity of was emphasized.
Zur（锌摄取调节剂）是 Fur（铁摄取调节剂）超家族的重要成员，广泛分布于细菌中。Zur 在锌稳态中发挥关键作用，并影响各种物种的细胞发育和环境适应。 是一种革兰氏阴性肠道病原体，通常作为致病性研究的模式生物。 Zur 对锌转运蛋白 ZnuABC 和蛋白分泌系统 T6SS 的调节作用已在 中得到证实。在这项研究中，使用 RNA-seq 对 的 ∆突变体和野生型（WT）菌株之间进行了比较转录组学分析。该分析揭示了 Zur 在包括膜转运、细胞运动以及分子和能量代谢在内的多个功能类别中的全局调节作用。此外， Zur 介导了不仅锌而且铁和镁的稳态。在 ∆突变体菌株中，有 35 个鞭毛生物合成和组装相关基因明显减少，导致游泳运动能力降低。此外， Zur 通过直接结合其启动子上调了多个简单糖和寡肽转运系统基因。 Zur 的缺失抑制了生物膜的形成，并降低了对氯霉素和酸性胁迫的抗性。这项研究说明了 Zur 的综合调节功能，强调了其在 中的应激抗性和致病性中的重要性。
细菌在环境中遇到多种压力，并且拥有必要的调节剂来调节基因的表达，以更好地适应压力并生存。 Zur（锌摄取调节剂）在锌稳态中发挥着重要作用。从多个物种综述的 Zur 研究表明，它影响细胞发育、应激抗性和细菌的毒力。 是一种肠道病原体，作为致病性、毒力因子和环境适应机制研究的模式生物。在这项研究中，在 中进行了 Zur 调控子的转录组学分析。 Zur 作为金属稳态、运动、营养物质获取、聚糖代谢和核苷酸代谢的全局调节剂的功能，反过来又增加了生物膜的形成、应激抗性和毒力。 Zur 在 的环境适应和致病性中的重要性得到了强调。
