Schütte Lina, Hausmann Katharina, Schwarz Christoph, Ersoy Franziska, Berger Ralf G
Institute of Food Chemistry, Gottfried Wilhelm Leibniz University Hannover, 30167 Hannover, Germany.
S2B GmbH & Co. KG, Zum Almsweg 2, 26197 Ahlhorn, Germany.
Bioengineering (Basel). 2024 Mar 16;11(3):284. doi: 10.3390/bioengineering11030284.
Following the idea of a circular bioeconomy, the use of side streams as substitutes for cultivation media (components) in bioprocesses would mean an enormous economic and ecological advantage. Costly compounds in conventional media for the production of the triterpene squalene in thraustochytrids are the main carbon source and complex nitrogen sources. Among other side streams examined, extracts from the spent mycelium of the basidiomycete were best-suited to acting as alternative nitrogen sources in cultivation media for thraustochytrids. The total nitrogen (3.76 ± 0.01 and 4.24 ± 0.04%, respectively) and protein (16.47 ± 0.06 and 18.57 ± 0.18%, respectively) contents of the fruiting body and mycelium were determined. The fungal cells were hydrolyzed and extracted to generate accessible nitrogen sources. Under preferred conditions, the extracts from the fruiting body and mycelium contained 73.63 ± 1.19 and 89.93 ± 7.54 mM of free amino groups, respectively. Cultivations of sp. S31 on a medium using a mycelium extract as a complex nitrogen source showed decelerated growth but a similar squalene yield (123.79 ± 14.11 mg/L after 216 h) compared to a conventional medium (111.29 ± 19.96 mg/L, although improvable by additional complex nitrogen source).
遵循循环生物经济的理念，在生物过程中使用副产物流作为培养介质（成分）的替代品将意味着巨大的经济和生态优势。在破囊壶菌中生产三萜角鲨烯的传统培养基中，昂贵的成分是主要碳源和复合氮源。在研究的其他副产物流中，担子菌废弃菌丝体的提取物最适合作为破囊壶菌培养基中的替代氮源。测定了子实体和菌丝体的总氮含量（分别为3.76±0.01%和4.24±0.04%）和蛋白质含量（分别为16.47±0.06%和18.57±0.18%）。对真菌细胞进行水解和提取以产生可利用的氮源。在优选条件下，子实体和菌丝体的提取物分别含有73.63±1.19 mM和89.93±7.54 mM的游离氨基。在使用菌丝体提取物作为复合氮源的培养基上培养sp. S31，与传统培养基相比，生长速度减慢，但角鲨烯产量相似（216小时后为123.79±14.11 mg/L）（传统培养基为111.29±19.96 mg/L，尽管添加额外的复合氮源可提高产量）。
