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抗病毒胡蜂毒液肽 Protopolybia-MP III 及其衍生物抗 HSV-1 的功能和机制。

Function and Mechanism of Antiviral Wasp Venom Peptide Protopolybia-MP III and Its Derivatives against HSV-1.

机构信息

Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, College of Basic Medicine, Hubei Key Laboratory of Embryonic Stem Cell Research, Hubei University of Medicine, Shiyan 442000, China.

Hubei Key Laboratory of Wudang Local Chinese Medicine Research, Hubei University of Medicine, Shiyan 442000, China.

出版信息

Toxins (Basel). 2024 Mar 4;16(3):132. doi: 10.3390/toxins16030132.

DOI:10.3390/toxins16030132
PMID:38535798
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10976043/
Abstract

Viruses are one of the leading causes of human disease, and many highly pathogenic viruses still have no specific treatment drugs. Therefore, producing new antiviral drugs is an urgent matter. In our study, we first found that the natural wasp venom peptide Protopolybia-MP III had a significant inhibitory effect on herpes simplex virus type 1 (HSV-1) replication by using quantitative real-time PCR (qPCR), Western blotting, and plaque-forming assays. Immunofluorescence analysis showed Protopolybia-MP III could enter cells, and it inhibited multiple stages of the HSV-1 life cycle, including the attachment, entry/fusion, and post-entry stages. Furthermore, ultracentrifugation and electron microscopy detected that Protopolybia-MP III significantly suppressed HSV-1 virion infectivity at different temperatures by destroying the integrity of the HSV-1 virion. Finally, by comparing the antiviral activity of Protopolybia-MP III and its mutants, a series of peptides with better anti-HSV-1 activity were identified. Overall, this work found the function and mechanism of the antiviral wasp venom peptide Protopolybia-MP III and its derivatives against HSV-1 and laid the foundation for the research and development of wasp venom-derived antiviral candidate peptide drugs.

摘要

病毒是导致人类疾病的主要原因之一，许多高致病性病毒仍然没有特定的治疗药物。因此，生产新的抗病毒药物是当务之急。在我们的研究中，我们首先发现天然胡蜂毒液肽 Protopolybia-MP III 通过定量实时 PCR（qPCR）、Western blot 和噬斑形成试验对单纯疱疹病毒 1（HSV-1）复制有显著抑制作用。免疫荧光分析表明 Protopolybia-MP III 可以进入细胞，并抑制 HSV-1 生命周期的多个阶段，包括附着、进入/融合和进入后阶段。此外，超速离心和电子显微镜检测到 Protopolybia-MP III 以不同的温度显著抑制 HSV-1 病毒粒子的感染力，破坏 HSV-1 病毒粒子的完整性。最后，通过比较 Protopolybia-MP III 及其突变体的抗病毒活性，鉴定出一系列具有更好抗 HSV-1 活性的肽。总的来说，这项工作发现了抗病毒胡蜂毒液肽 Protopolybia-MP III 及其衍生物对 HSV-1 的功能和作用机制，为研究和开发胡蜂毒液衍生的抗病毒候选肽药物奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70e6/10976043/a459f60b784b/toxins-16-00132-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70e6/10976043/6ca890e0cb44/toxins-16-00132-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70e6/10976043/83edc16307a6/toxins-16-00132-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70e6/10976043/4b2539f108c4/toxins-16-00132-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70e6/10976043/7fca31724da9/toxins-16-00132-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70e6/10976043/753071a8207c/toxins-16-00132-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70e6/10976043/28bb33fa31be/toxins-16-00132-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70e6/10976043/a459f60b784b/toxins-16-00132-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70e6/10976043/6ca890e0cb44/toxins-16-00132-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70e6/10976043/83edc16307a6/toxins-16-00132-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70e6/10976043/4b2539f108c4/toxins-16-00132-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70e6/10976043/7fca31724da9/toxins-16-00132-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70e6/10976043/753071a8207c/toxins-16-00132-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70e6/10976043/28bb33fa31be/toxins-16-00132-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/70e6/10976043/a459f60b784b/toxins-16-00132-g007.jpg

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