Tivers Michael S, Mirczuk Samantha M, Charlesworth Abigail, Wood Lauren, Barker Emi N, Lipscomb Victoria J, Fowkes Robert C
Paragon Veterinary Referrals, Paragon Business Village, Red Hall Cres, Wakefield WF1 2DF, UK.
Langford Vets, University of Bristol, Langford BS40 5DU, UK.
Vet Sci. 2024 Feb 26;11(3):100. doi: 10.3390/vetsci11030100.
Congenital portosystemic shunts (CPSS) are vascular anomalies resulting in liver hypoplasia and hepatic insufficiency. Cats with CPSS typically show signs of hepatic encephalopathy associated with increased ammonia, inflammatory cytokines, and oxidative stress. Surgical attenuation of the CPSS results in improved liver function, resolution of clinical signs, and increased portal blood flow. Hepatic gene expression has not previously been investigated in cats with CPSS. Here, we compared the hepatic expression of genes involved in the urea cycle (, ), angiogenesis (, , , , , ), liver regeneration (, , ), and metabolism () from a small series of cats ( = 18) with CPSS to that of control cats ( = 10). The expression of , , , , and was significantly elevated in liver biopsies from cats with CPSS. Cats that could only tolerate partial closure of their CPSS had increased hepatic expression of , , and compared with those that could tolerate complete ligation. Furthermore, there were no significant correlations between gene expression and pre-operative plasma ammonia concentrations in cats with CPSS. The changes in hepatic gene expression in cats with CPSS are in direct contrast to those seen in dogs with CPSS, suggesting alternative mechanisms may be involved in mediating hepatic changes in cats with CPSS.
先天性门体分流（CPSS）是导致肝脏发育不全和肝功能不全的血管异常。患有CPSS的猫通常表现出与氨、炎性细胞因子和氧化应激增加相关的肝性脑病迹象。对CPSS进行手术减流可改善肝功能、消除临床症状并增加门静脉血流量。此前尚未对患有CPSS的猫的肝脏基因表达进行研究。在此，我们比较了一小群患有CPSS的猫（n = 18）与对照猫（n = 10）中参与尿素循环（、）、血管生成（、、、、）、肝脏再生（、、）和代谢（）的基因的肝脏表达情况。在患有CPSS的猫的肝脏活检中，、、、和的表达显著升高。与能够耐受完全结扎的猫相比，只能耐受CPSS部分闭合的猫的肝脏中、和的表达增加。此外，患有CPSS的猫的基因表达与术前血浆氨浓度之间没有显著相关性。患有CPSS的猫的肝脏基因表达变化与患有CPSS的狗的情况形成直接对比，表明可能涉及其他机制来介导患有CPSS的猫的肝脏变化。
