Department of Neurosurgery, 3201 Hospital of Xi'an Jiaotong University Health Science Center, 723000 Hanzhong, Shaanxi, China.
Department of Pathology, 3201 Hospital of Xi'an Jiaotong University Health Science Center, 723000 Hanzhong, Shaanxi, China.
Front Biosci (Landmark Ed). 2024 Mar 15;29(3):104. doi: 10.31083/j.fbl2903104.
World Health Organization (WHO) grade 4 adult-type diffuse glioma is the most malignant primary tumor of the brain. Nucleolar protein 14 () is recognized to contribute significantly to the assembly of small ribosomal subunits. However, the specific involvement of NOP14 in diverse cancers remains poorly understood, particularly its role in adult-type diffuse glioma, which has yet to be elucidated.
A total of 20 adult-type diffuse glioma samples with varying WHO stages were collected. The protein level of NOP14 was detected using immunohistochemistry. Additionally, NOP14 expression in LN229 and U251 cell lines and collected clinical tissue samples was quantified using the Western blot technique. Furthermore, the correlation between and clinicopathological features, survival rates, matrix and immune scores, and immune components was investigated using data from the Cancer Gene Atlas database.
exhibited high expression in adult-type diffuse glioma patients, with the highest expression observed in the LN229 cell line. Moreover, elevated expression was significantly correlated with poorer overall survival and demonstrated an association with unfavorable pathological features in a cohort of 703 glioblastoma (GBM) patients. Evidence of a connection between and the tumor microenvironment was presented. Elevated was linked to the infiltration of CD8+T cell and factors related to epithelial-mesenchymal transition. In assay, was capable of suppressing adult-type diffuse glioma cell invasion and metastasis.
holds great promise as a candidate biomarker for detecting prognostic, molecular, and immune signatures of adult-type diffuse glioma.
世界卫生组织（WHO）4 级成人弥漫性神经胶质瘤是大脑中最恶性的原发性肿瘤。核仁蛋白 14（）被认为对小核糖体亚基的组装有重要贡献。然而，NOP14 在不同癌症中的具体作用仍知之甚少，特别是其在成人弥漫性神经胶质瘤中的作用尚未阐明。
共收集了 20 例不同 WHO 级别的成人弥漫性神经胶质瘤样本。采用免疫组织化学法检测 NOP14 的蛋白水平。此外，采用 Western blot 技术定量检测 LN229 和 U251 细胞系和收集的临床组织样本中的 NOP14 表达。此外，还利用癌症基因图谱数据库的数据研究了与临床病理特征、生存率、基质和免疫评分以及免疫成分之间的相关性。
在成人弥漫性神经胶质瘤患者中，表达水平较高，在 LN229 细胞系中表达最高。此外，升高的表达水平与总生存率降低显著相关，并与 703 例胶质母细胞瘤（GBM）患者的不良病理特征相关。还提出了证据表明与肿瘤微环境之间存在联系。升高的与 CD8+T 细胞浸润和上皮-间充质转化相关因子有关。在细胞侵袭和转移实验中，能够抑制成人弥漫性神经胶质瘤细胞的侵袭和转移。
作为检测成人弥漫性神经胶质瘤预后、分子和免疫特征的候选生物标志物，具有很大的潜力。
