Jakubowicz T, Frielle D W, Vaughan M H
Acta Biochim Pol. 1985;32(3):199-210.
A significant, reversible decline in the level of 40S subunit X Met-tRNAf complexes was observed in HeLa cells treated with either of two inhibitors of tRNA charging, histidinol or O-methylthreonine. A decline in 40S X subunit Met-tRNAf complexes was also seen in HeLa cells deprived of histidine and in mutant Chinese hamster ovary cells, bearing a temperature-sensitive leucyl-tRNA charging enzyme, when they were incubated at a non-permissive temperature. Treatment with histidinol or O-methylthreonine did not affect the relative degree of phosphorylation of the alpha subunit of initiation factor eIF-2. Histidinol did not stimulate the activity of the endogenous kinase for eIF-2 alpha subunit in extracts prepared from HeLa cells. In the presence of histidinol the phosphorylation level of a 25000-dalton protein was reversibly increased.
在用两种tRNA充电抑制剂（组氨醇或O-甲基苏氨酸）之一处理的HeLa细胞中，观察到40S亚基X Met-tRNAf复合物水平出现显著的、可逆的下降。在缺乏组氨酸的HeLa细胞以及携带温度敏感型亮氨酰-tRNA充电酶的突变中国仓鼠卵巢细胞中，当它们在非允许温度下孵育时，也观察到40S X亚基Met-tRNAf复合物的下降。用组氨醇或O-甲基苏氨酸处理并不影响起始因子eIF-2α亚基的相对磷酸化程度。组氨醇不会刺激从HeLa细胞制备的提取物中内源性eIF-2α亚基激酶的活性。在组氨醇存在的情况下，一种25000道尔顿蛋白质的磷酸化水平可逆地增加。
