Center for Computational and Integrative Biology, Rutgers The State University of New Jersey, Joint Health Sciences Center, 201 South Broadway, Camden, NJ 08103, USA.
Department of Aquaculture and Fish Diseases, Fisheries Faculty, Firat University, 23200 Elazig, Türkiye.
Genes (Basel). 2024 Feb 24;15(3):283. doi: 10.3390/genes15030283.
The glossiphoniid leech, , is an experimentally tractable member of the superphylum, Lophotrochozoa. Its large embryonic cells, stereotyped asymmetric cell divisions and ex vivo development capabilities makes it a favorable model for studying the molecular and cellular events of a representative spiralian. In this study, we focused on a narrow developmental time window of 6-8 h, comprising stages just prior to and immediately following zygote deposition. Employing RNA-Seq methodology, we identified differentially expressed transcripts at this fundamental ontogenic boundary, known as the maternal-to-zygotic transition (MZT). Gene expression changes were characterized by the massive degradation of maternal RNAs (45%) coupled with the rapid transcription of 5000 zygotic genes (20% of the genome) in the first mitotic cell cycle. The latter transcripts encoded a mixture of cell maintenance and regulatory proteins that predictably influence downstream developmental events.
豆齿舌蛭， ，是超门，担轮动物的一个可实验操作的成员。它的大胚胎细胞、定型的不对称细胞分裂和离体发育能力使其成为研究代表性螺旋动物的分子和细胞事件的理想模型。在这项研究中，我们集中在一个狭窄的发育时间窗口约 6-8 小时，包括在受精卵沉积之前和之后的阶段。我们采用 RNA-Seq 方法，在这个被称为母体到合子过渡 (MZT) 的基本个体发育边界处鉴定出差异表达的转录本。基因表达变化的特点是大量降解母体 RNA（约 45%），同时在第一次有丝分裂细胞周期中快速转录约 5000 个合子基因（约基因组的 20%）。后者的转录本编码了一种细胞维持和调节蛋白的混合物，可预测性地影响下游的发育事件。
