Wu Shuanghui, Niu Yin, Wang Jie, Dao Xiaofang, Lin Yaqiu, Chen Juan
College of Food Science and Technology, Southwest Minzu University, Chengdu 610225, China.
College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China.
Foods. 2024 Mar 7;13(6):817. doi: 10.3390/foods13060817.
The influence of different levels of sodium chloride, sodium nitrite, and glucose on the quality characteristics of spontaneously fermented goat meat sausage was investigated. The amounts of total biogenic amines in all the sausages ranged from 324.70 to 388.77 mg/kg; among them, spermine was the most abundant, with amounts ranging from 230.96 to 275.78 mg/kg. Increasing sodium chloride from 15 to 35 g/kg, the content of cadaverine, putrescine, tyramine, phenylethylamine, tryptamine, and total amines decreased, and Enterobacteriaceae counts decreased at the same time. Increasing sodium nitrite from 150 to 250 mg/kg, the content of cadaverine, histamine, and total amines decreased, while Enterobacteriaceae counts decreased simultaneously. Increasing glucose from 10 to 40 g/kg, the content of cadaverine, spermidine, and total amines decreased. was the most abundant genus across all the samples, and the relative abundance of was reduced obviously by increasing sodium nitrite and glucose levels. The top 10 differential bacterial taxa for each additive group were respectively obtained, and microbial biomarkers for each level of additive within its group were acquired, respectively. Through Pearson correlation, was positively correlated with phenylethylamine, tryptamine, tyramine, and cadaverine, and were positively correlated with phenylethylamine and putrescine, respectively, suggesting they have the potential to produce biogenic amines. The results provided references for controlling the accumulation of biogenic amines in fermented goat meat sausage via the addition of auxiliary additives during the processing.
研究了不同水平的氯化钠、亚硝酸钠和葡萄糖对自发发酵山羊肉香肠品质特性的影响。所有香肠中总生物胺含量在324.70至388.77毫克/千克之间;其中,精胺含量最高,在230.96至275.78毫克/千克之间。将氯化钠从15克/千克增加到35克/千克,尸胺、腐胺、酪胺、苯乙胺、色胺和总胺的含量降低,同时肠杆菌科数量减少。将亚硝酸钠从150毫克/千克增加到250毫克/千克,尸胺、组胺和总胺的含量降低,同时肠杆菌科数量也减少。将葡萄糖从10克/千克增加到40克/千克,尸胺、亚精胺和总胺的含量降低。 是所有样品中最丰富的属,通过增加亚硝酸钠和葡萄糖水平, 的相对丰度明显降低。分别获得了每个添加剂组的前10个差异细菌分类群,并分别获得了其组内每个添加剂水平的微生物生物标志物。通过Pearson相关性分析, 与苯乙胺、色胺、酪胺和尸胺呈正相关, 和 分别与苯乙胺和腐胺呈正相关,表明它们具有产生生物胺的潜力。该结果为在加工过程中通过添加辅助添加剂控制发酵山羊肉香肠中生物胺的积累提供了参考。