Evaluation of Cellular Responses of Heterotrophic Cultured with Autotrophic as a Nutrient Source by Analyses Based on Microbiology and Transcriptome.

Heterotrophic microorganism LS5218 was cultured with flesh green alga C-9: NIES-2235 as a nutrient supplier. In order to evaluate the cell response of with , was evaluated with microbial methods and comprehensive gene transcriptional analyses. with showed a specific growth rate () of 1.04 ± 0.27, which was similar to that for cells growing in Luria-Bertani medium ( = 1.20 ± 0.40 h). Furthermore, comparing the cellular responses of in a green-algae-containing medium with those in the Luria-Bertani medium, transcriptomic analysis showed that upregulated gene transcription levels related to glycolysis, 5-phospho-d-ribosyl-1-diphosphate, and lipid synthesis; on the other hand, it decreased the levels related to lipid degradation. In particular, the transcription levels were increased by 103.7 times on ( * < 0.05 ( = 0.015)) in glycolysis, and decreased by 0.247 times on ( * < 0.05 ( = 0.041)) in lipolysis. These genes are unique and could regulate the direction of metabolism; these responses possibly indicate carbon source assimilation as a cellular response in . This paper is the first report to clarify that , a substance-producing strain, directly uses as a nutrient supplier by evaluation of the cellular responses analyzed with microbial methods and transcriptome analysis.