Nakanishi Akihito, Omino Natsumi, Nakamura Tomoyo, Goto Saki, Matsumoto Riri, Yomogita Misaki, Narisawa Naoki, Kimijima Manami, Iritani Kohei
School of Bioscience and Biotechnology, Tokyo University of Technology, Hachioji 192-0982, Japan.
Graduate School of Bionics, Tokyo University of Technology, Hachioji 192-0982, Japan.
Microorganisms. 2024 Feb 23;12(3):452. doi: 10.3390/microorganisms12030452.
Heterotrophic microorganism LS5218 was cultured with flesh green alga C-9: NIES-2235 as a nutrient supplier. In order to evaluate the cell response of with , was evaluated with microbial methods and comprehensive gene transcriptional analyses. with showed a specific growth rate () of 1.04 ± 0.27, which was similar to that for cells growing in Luria-Bertani medium ( = 1.20 ± 0.40 h). Furthermore, comparing the cellular responses of in a green-algae-containing medium with those in the Luria-Bertani medium, transcriptomic analysis showed that upregulated gene transcription levels related to glycolysis, 5-phospho-d-ribosyl-1-diphosphate, and lipid synthesis; on the other hand, it decreased the levels related to lipid degradation. In particular, the transcription levels were increased by 103.7 times on ( * < 0.05 ( = 0.015)) in glycolysis, and decreased by 0.247 times on ( * < 0.05 ( = 0.041)) in lipolysis. These genes are unique and could regulate the direction of metabolism; these responses possibly indicate carbon source assimilation as a cellular response in . This paper is the first report to clarify that , a substance-producing strain, directly uses as a nutrient supplier by evaluation of the cellular responses analyzed with microbial methods and transcriptome analysis.
以肉色绿藻C-9:NIES-2235作为营养供应源培养异养微生物LS5218。为了评估其细胞反应，采用微生物方法和综合基因转录分析对其进行了评估。其比生长速率（μ）为1.04±0.27，与在Luria-Bertani培养基中生长的细胞（μ = 1.20±0.40 h⁻¹）相似。此外，通过转录组分析比较含绿藻培养基和Luria-Bertani培养基中其细胞反应，结果显示其上调了与糖酵解、5-磷酸-d-核糖-1-二磷酸和脂质合成相关的基因转录水平；另一方面，降低了与脂质降解相关的水平。特别是，糖酵解中相关转录水平在某物质上增加了103.7倍（P*<0.05（P = 0.015）），脂解中相关转录水平在某物质上降低了0.247倍（P*<0.05（P = 0.041））。这些基因是独特的，可调节代谢方向；这些反应可能表明其作为细胞反应的碳源同化作用。本文首次通过微生物方法和转录组分析评估细胞反应，阐明了产物质菌株直接利用绿藻作为营养供应源。
