Skwor Troy, Jones Dan Christopher, Cahak Caitlin, Newton Ryan J
School of Biomedical Sciences and Health Care Administration, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USA.
Wisconsin Diagnostics Laboratory, Milwaukee, WI 53226, USA.
Microorganisms. 2024 Feb 29;12(3):494. doi: 10.3390/microorganisms12030494.
Antibiotic resistance remains one of the most pressing public health issues facing the world today. At the forefront of this battle lies the ever-increasing identification of extended-spectrum beta-lactamases and carbapenemases within human pathogens, conferring resistance towards broad-spectrum and last-resort antimicrobials. This study was prompted due to the identification of a pathogenic isolate (strain MAH-4) collected from abdominal fluid, which presented a robust resistance pattern against second-, third-, and fourth-generation cephalosporins, ertapenem, ciprofloxacin, gentamicin, levofloxacin and moxifloxacin, and beta lactam/beta-lactamase inhibitor combinations. Whole genome sequencing was performed and identified a 328 kb plasmid (pMAH4) encoding 10 antibiotic resistance genes, including , , and of MAH-4. This is the first report of beta-lactamase SFO-1 within a clinical strain of . Due to the remarkable sequence identity of pMAH4 to plasmids associated with genera like and the extensive capabilities of for horizontal gene transfer, our identification of a clinical isolate encoding SFO-1 on a plasmid suggests antibiotic resistance gene mobility between and non- species.
抗生素耐药性仍然是当今世界面临的最紧迫的公共卫生问题之一。这场斗争的前沿是在人类病原体中不断发现超广谱β-内酰胺酶和碳青霉烯酶，这些酶使病原体对广谱和最后手段的抗菌药物产生耐药性。本研究是由于从腹腔积液中分离出一株致病菌株（菌株MAH-4）而引发的，该菌株对第二代、第三代和第四代头孢菌素、厄他培南、环丙沙星、庆大霉素、左氧氟沙星和莫西沙星以及β-内酰胺/β-内酰胺酶抑制剂组合呈现出强大的耐药模式。进行了全基因组测序，鉴定出一个328 kb的质粒（pMAH4），其编码10个抗生素耐药基因，包括MAH-4的、和。这是β-内酰胺酶SFO-1在临床菌株中的首次报道。由于pMAH4与、等属相关质粒具有显著的序列同一性，且具有广泛的水平基因转移能力，我们在质粒上鉴定出编码SFO-1的临床分离株，这表明抗生素耐药基因在和非物种之间具有流动性。
