Dupont François, Stoukatch Serguei, Laurent Philippe, Eersels Kasper, van Grinsven Bart, Redouté Jean-Michel
Microsys Laboratory, Department of Electrical Engineering and Computer Science, University of Liège, 4000 Liège, Belgium.
Sensor Engineering Department, Faculty of Science and Engineering, Maastricht University, 6200 MD Maastricht, The Netherlands.
Sensors (Basel). 2024 Mar 19;24(6):1960. doi: 10.3390/s24061960.
Colorimetric characterisation systems based on LEDs and RBG sensors are straightforward to implement, are highly integrable allowing for portable measurement systems and can be constructed using widespread and affordable components. They have already proved to be a satisfactory solution in several applications related to chemical analysis. In this paper, we present an RGB sensor-based prototype for colorimetric characterisation, which can accommodate cuvettes with optical paths of 10 mm and 40 mm. We assessed the impact of experimental condition parameters such as the variability of the analyte volume in the cuvette, as well as the presence of floating particles or deposits at the bottom of the cuvette. While these would not impact the result given by a spectrophotometer that generally has a directional light source, they must be considered in LED/RGB sensor analysers in which the light path is not tightly controlled. We demonstrated that there is a minimal sensor height above the bottom of the cuvette and a minimal analyte level (both depending on the prototype optical path length) above which the analyte volume and the presence of floating particles and deposits have no impact on the prototype output signal. Finally, based on these results, we proposed a test method for a quick dye-displacement assay, in which the reagent is a dye-loaded molecularly imprinted polymer that is poured directly into a cuvette.
基于发光二极管（LED）和红、绿、蓝（RGB）传感器的比色表征系统易于实现，具有高度集成性，可用于便携式测量系统，并且可以使用广泛且价格低廉的组件构建。它们已被证明是在与化学分析相关的多个应用中令人满意的解决方案。在本文中，我们展示了一种基于RGB传感器的比色表征原型，它可以容纳光程为10毫米和40毫米的比色皿。我们评估了实验条件参数的影响，例如比色皿中分析物体积的变化，以及比色皿底部存在的漂浮颗粒或沉积物。虽然这些不会影响通常具有定向光源的分光光度计给出的结果，但在光程不受严格控制的LED/RGB传感器分析仪中必须予以考虑。我们证明，在比色皿底部上方存在最小传感器高度，并且在分析物水平高于该高度时（两者均取决于原型光程长度），分析物体积以及漂浮颗粒和沉积物的存在对原型输出信号没有影响。最后，基于这些结果，我们提出了一种用于快速染料置换测定的测试方法，其中试剂是直接倒入比色皿中的负载染料的分子印迹聚合物。
