Dorikhani Arezoo, Omidi Ameneh, Movahedin Mansoureh, Halvaei Iman
Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Int J Reprod Biomed. 2024 Feb 23;22(1):43-54. doi: 10.18502/ijrm.v22i1.15241. eCollection 2024 Jan.
Due to myelin and axonal insults in multiple sclerosis individuals, motor coordination problems and endocrine imbalance may develop.
This study aims to evaluate the role of chronic demyelination on the hypothalamic-pituitary-gonadal axis in the mouse model of multiple sclerosis.
20 adult C57/BL6 male mice were divided into 2 groups (n = 10/each) as follows: the control group (CONT) received a regular diet for 17 wk; and the experimental group (cuprizone [CPZ]) was fed with 0.2% CPZ for 12 wk and, then CPZ was withdrawn for 5 wk. Serum testosterone, histopathology of the brain and testis, and sperm analysis were evaluated.
The hypothalamic myelin content was significantly decreased in the arcuate nucleus following the 12 wk of CPZ consumption compared to the CONT group, and the statistical difference remained until 17 wk. Testosterone levels declined significantly in the CPZ group compared to the CONT group in the 12 and 17 wk. A significant decrease was observed in the height of the seminiferous epithelium and the interstitial tissue area, and the number of seminiferous epithelial cells in the CPZ group compared to the CONT group in the 12 and 17 wk. The sperm count, motility, and viability in the CPZ group significantly decreased compared to the CONT group in the 12 and 17 wk of the study.
Chronic demyelination induced by CPZ intoxication, maybe through damage to the hypothalamus arcuate nucleus, leads to the hypothalamic-pituitary-gonadal axis disturbance and damage to the testis and spermatogenesis subsequently.
由于多发性硬化症患者存在髓鞘和轴突损伤，可能会出现运动协调问题和内分泌失调。
本研究旨在评估慢性脱髓鞘在多发性硬化症小鼠模型中对下丘脑 - 垂体 - 性腺轴的作用。
将20只成年C57/BL6雄性小鼠分为2组（每组n = 10），如下：对照组（CONT）接受常规饮食17周；实验组（曲吡酮[CPZ]组）给予0.2% CPZ喂养12周，然后撤去CPZ 5周。评估血清睾酮、脑和睾丸的组织病理学以及精子分析。
与CONT组相比，CPZ摄入12周后，弓状核的下丘脑髓鞘含量显著降低，且这种统计学差异一直持续到17周。在12周和17周时，CPZ组的睾酮水平与CONT组相比显著下降。与CONT组相比，在12周和17周时，CPZ组的生精上皮高度、间质组织面积以及生精上皮细胞数量均显著减少。在研究的12周和17周时，CPZ组的精子计数、活力和存活率与CONT组相比显著降低。
CPZ中毒诱导的慢性脱髓鞘可能通过损伤下丘脑弓状核，导致下丘脑 - 垂体 - 性腺轴紊乱，进而损伤睾丸和精子发生。
