Zhu Lingxuan, Cui Xin, Hu Shantong, Wang Shihui, Zhang Guimin
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
Sheng Wu Gong Cheng Xue Bao. 2024 Mar 25;40(3):834-846. doi: 10.13345/j.cjb.230512.
The signal peptide is a key factor that affects the efficiency of protein secretion in . Currently, the most used signal peptide is the α-mating factor (MFα) pre-pro leader from . This exogenous signal peptide has been successfully utilized to express and secret many heterologous proteins. However, MFα is not suitable for the secretory expression of all heterologous proteins. Many typical signal peptides are present in the secretory proteins of . , which provides more options besides MFα. Therefore, it is necessary to analyze and identify more efficient endogenous signal peptides that can guide the secretion of heterologous proteins in . . In this study, we employed bioinformatics tools such as SignalP, TMHMM, Phobius, WoLF PSORT, and NetGPI to predict endogenous signal peptides from the entire proteome of . GS115 (ATCC 20864). Moreover, we analyzed the distribution, length, amino acid composition, and conservation of these signal peptides. Additionally, we screened 69 secreted proteins and their signal peptides, and through secretome validation, we identified 10 endogenous signal peptides that have potential to be used for exogenous protein expression. The endogenous signal peptides obtained in this study may serve as new valuable tools for the expression and secretion of heterologous proteins in . .
信号肽是影响蛋白质分泌效率的关键因素。目前，最常用的信号肽是来自酿酒酵母的α-交配因子（MFα）前原导肽。这种外源信号肽已成功用于表达和分泌许多异源蛋白。然而，MFα并不适用于所有异源蛋白的分泌表达。酿酒酵母的分泌蛋白中存在许多典型的信号肽，这为除MFα之外提供了更多选择。因此，有必要分析和鉴定更多能够指导酿酒酵母中异源蛋白分泌的高效内源性信号肽。在本研究中，我们使用了SignalP、TMHMM、Phobius、WoLF PSORT和NetGPI等生物信息学工具从酿酒酵母GS115（ATCC 20864）的整个蛋白质组中预测内源性信号肽。此外，我们分析了这些信号肽的分布、长度、氨基酸组成和保守性。另外，我们筛选了69种分泌蛋白及其信号肽，并通过分泌蛋白质组验证，鉴定出10种具有用于外源蛋白表达潜力的内源性信号肽。本研究中获得的内源性信号肽可能成为酿酒酵母中异源蛋白表达和分泌的新的有价值工具。
