Gilewski Anne L, Flower Jennifer E, Miller Lisa, Tuttle Allison D
Mystic Aquarium, A Division of Sea Research Foundation, Mystic, Connecticut, USA.
Companion Animal Health, New Castle, Delaware, USA.
Zoo Biol. 2024 Jul-Aug;43(4):376-382. doi: 10.1002/zoo.21832. Epub 2024 Mar 28.
Photobiomodulation therapy (PBMT) has become increasingly utilized in the zoo and aquarium fields to treat ailments in various species; however, its use in teleosts and elasmobranchs is relatively underrepresented in the literature. This study examined the efficacy of PBMT as an adjunct treatment to reduce the healing time of dermal abrasions in cownose rays (Rhinoptera bonasus). Ten cownose rays were included in this study and separated into control (n = 5) and treatment groups (n = 5). Animals in both treatment and control groups received intramuscular injections of enrofloxacin every 72 h to provide broad-spectrum antibiotic coverage. Cownose rays in the treatment group additionally received PBMT using the Companion® CTC-12 unit using the large, noncontact head. Control group rays were subjected to identical handling without the use of PBMT. A total of 196 Joules were delivered at a power of 2 W based on a dose of 5 J/cm at each session. Observational analysis revealed a lack of appreciable difference in healing time between the treatment and control groups in the aforementioned settings; however, it is recommended that PBMT protocols should be re-evaluated if there is little to no response in healing after three to four sessions. PBMT use on acute superficial dermal abrasions in cownose rays was well-tolerated in this study and may have utility in a multimodal treatment approach to wound healing. This study also produced a wound scoring metric based on photographs captured at each treatment. Future studies should utilize increased fluence (J/cm) and irradiance (W/cm) and incorporate synchronous histological analysis and increased sample size.
光生物调节疗法（PBMT）在动物园和水族馆领域越来越多地被用于治疗各种物种的疾病；然而，其在硬骨鱼和软骨鱼中的应用在文献中相对较少。本研究考察了PBMT作为辅助治疗手段对减少牛鼻鲼（Rhinoptera bonasus）皮肤擦伤愈合时间的效果。本研究纳入了10条牛鼻鲼，并将其分为对照组（n = 5）和治疗组（n = 5）。治疗组和对照组的动物均每72小时接受一次恩诺沙星肌肉注射，以提供广谱抗生素覆盖。治疗组的牛鼻鲼还使用Companion® CTC - 12设备的大型非接触式探头接受PBMT治疗。对照组的牛鼻鲼接受相同的处理，但不使用PBMT。每次治疗基于5 J/cm²的剂量以2 W的功率总共输送196焦耳。观察分析显示，在上述设置下，治疗组和对照组在愈合时间上没有明显差异；然而，如果在三到四次治疗后愈合几乎没有反应，建议重新评估PBMT方案。在本研究中，PBMT用于牛鼻鲼急性浅表皮肤擦伤耐受性良好，可能在伤口愈合的多模式治疗方法中具有实用价值。本研究还根据每次治疗时拍摄的照片制定了伤口评分标准。未来的研究应使用更高的能量密度（J/cm²）和辐照度（W/cm²），并纳入同步组织学分析和增加样本量。
