College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, China.
Department of Medical college, Medical College of Yan'an University, Yan'an, China.
J Basic Microbiol. 2024 Jul;64(7):e2400008. doi: 10.1002/jobm.202400008. Epub 2024 Mar 28.
Arthrobotrys flagrans, a nematode-eating fungus, is an effective component of animal parasitic nematode biocontrol agents. In the dried formulation, the majority of spores are in an endogenous dormant state. This study focuses on dormant chlamydospore and nondormant chlamydospore of A. flagrans to investigate the differences in cyclic adenosine monophosphate (cAMP) and protein content between the two types of spores. cAMP and soluble proteins were extracted from the nondormant chlamydospore and dormant chlamydospore of two isolates of A. flagrans. The cAMP Direct Immunoassay Kit and Bradford protein concentration assay kit (Coomassie brilliant blue method) were used to detect the cAMP and protein content in two types of spores. Results showed that the content of cAMP in dormant spores of both isolates was significantly higher than that in nondormant spores (p < 0.05). The protein content of dormant spores in DH055 bacteria was significantly higher than that of nondormant spores (p < 0.05). In addition, the protein content of dormant spores of the SDH035 strain was slightly higher than that of nondormant spores, but the difference was not significant (p > 0.05). The results obtained in this study provide evidence for the biochemical mechanism of chlamydospore dormancy or the germination of the nematophagous fungus A. flagrans.
艳丽枝孢菌，一种捕食线虫的真菌，是动物寄生性线虫生物防治剂的有效成分。在干燥制剂中，大多数孢子处于内源性休眠状态。本研究聚焦于艳丽枝孢菌的休眠厚垣孢子和非休眠厚垣孢子，以研究这两种孢子之间环腺苷酸（cAMP）和蛋白质含量的差异。从两株艳丽枝孢菌的非休眠厚垣孢子和休眠厚垣孢子中提取 cAMP 和可溶性蛋白质。使用 cAMP 直接免疫测定试剂盒和 Bradford 蛋白浓度测定试剂盒（考马斯亮蓝法）检测两种孢子中的 cAMP 和蛋白质含量。结果表明，两株菌休眠孢子中的 cAMP 含量明显高于非休眠孢子（p<0.05）。DH055 细菌休眠孢子中的蛋白质含量明显高于非休眠孢子（p<0.05）。此外，SDH035 菌株休眠孢子中的蛋白质含量略高于非休眠孢子，但差异不显著（p>0.05）。本研究结果为艳丽枝孢菌厚垣孢子休眠或萌发的生化机制提供了证据。
