M.V. Lomonosov Moscow State University, Moscow, Russia.
Methods Mol Biol. 2024;2758:61-75. doi: 10.1007/978-1-0716-3646-6_3.
Natural peptides secreted under stress conditions by many organisms are bioactive molecules with a broad spectrum of activities. These molecules could become potential models for novel pharmaceuticals, to which bacteria, according to modern scientific concepts, do not have and cannot develop resistance. Taking this into consideration, it is necessary to clarify the amino acid sequences of such peptides. Here we describe our approach to de novo sequencing of amphibians' skin secretion peptides.
在应激条件下，许多生物体分泌的天然肽是具有广泛活性的生物活性分子。这些分子可能成为新型药物的潜在模型，根据现代科学概念，细菌没有也不能对这些药物产生耐药性。考虑到这一点，有必要阐明这些肽的氨基酸序列。在这里，我们描述了一种从头测序两栖动物皮肤分泌物肽的方法。
