School of Biological and Pharmaceutical Engineering, Lanzhou Jiao tong University, Lanzhou 730070, Gansu, China.
Key Laboratory of Biodiversity Formation Mechanism and Comprehensive Utilization of the Qinghai-Tibet Plateau in Qinghai Province, Qinghai Normal University, Xining 810008, China.
Plant Dis. 2024 Aug;108(8):2503-2517. doi: 10.1094/PDIS-10-23-2178-RE. Epub 2024 Aug 8.
Root rot is a very destructive soil-borne disease, which severely affects the quality and yield of in major planting areas of Gansu Province, China. Twelve strains were identified from root rot tissue and infected soil in the field by comparing each isolate strain internal transcribed spacer, translation elongation factor 1-α sequence and RNA polymerase second largest subunit gene with the sequences of known fungal species in the NCBI database. Of these isolates, four were , followed by three , two , and one each of , , and . Under greenhouse conditions, pathogenicity testing experiment was carried out using five strains: two , one , one , and one . Among them, the incidence of -induced root rot on was 100%; hence, it was the most aggressive. Liquid chromatography was used to show that could produce neosolaniol (NEO), deoxynivalenol, and T-2 toxins. Of these, the level of NEO produced by was high compared with the other two toxins. By isolating spp. and characterizing their toxin-producing capacity, this work provides new information for effectively preventing and controlling root rot in the field as well as improving the quality of its medicinal materials.
根腐病是一种极具破坏性的土传病害，严重影响中国甘肃省主要种植区的 质量和产量。通过比较每个分离株的内转录间隔区、翻译延伸因子 1-α 序列和 RNA 聚合酶第二大亚基基因与 NCBI 数据库中已知真菌种的序列，从田间根腐组织和感染土壤中鉴定出 12 株 。这些分离株中， 有 4 株，其次是 3 株、2 株、1 株 、 、 。在温室条件下，用 5 株菌进行致病性试验：2 株 、1 株 、1 株 、1 株 。其中， 引起 的根腐病发病率为 100%；因此，它的攻击性最强。液相色谱法显示 能够产生新茄病镰刀菌醇（NEO）、脱氧雪腐镰刀菌烯醇和 T-2 毒素。其中， 产生的 NEO 水平高于其他两种毒素。通过分离 和表征其产毒能力，这项工作为有效防治田间 根腐病以及提高其药材质量提供了新的信息。
