Haubeck H D, Kölsch E, Imort M, Hasilik A, von Figura K
J Immunol. 1985 Jan;134(1):65-9.
Interaction of mannose 6-phosphate-specific receptors with their ligands has been suggested to be essential for natural killer cell (NK)-mediated cytotoxicity. Indeed, mannose 6-phosphate-specific receptors and ligands bearing mannose 6-phosphate residues are demonstrable on human peripheral blood leukocytes with NK activity as well as on K-562 NK target cells, allowing at least in principle such an interaction. It can also be shown that NK activity of human peripheral blood leukocytes is inhibited by mannose 6-phosphate. The following observations, however, exclude an essential role of the mannose 6-phosphate receptor-ligand system in NK cell-mediated cytotoxicity. 1) NK cytotoxicity is sensitive to a broad range of structurally unrelated sugar phosphates. 2) NK activity is normal in patients with I cell disease (mucolipidosis II), which due to a genetic defect are unable to synthesize the ligands for the mannose 6-phosphate-specific receptor. 3) NK cytotoxicity is not inhibited by an antiserum against the mannose 6-phosphate receptor, which blocks the receptor function.
已有人提出，6-磷酸甘露糖特异性受体与其配体的相互作用对于自然杀伤细胞（NK）介导的细胞毒性至关重要。事实上，在具有NK活性的人外周血白细胞以及K-562 NK靶细胞上都可检测到6-磷酸甘露糖特异性受体和带有6-磷酸甘露糖残基的配体，这至少在原则上允许这种相互作用。还可以证明，6-磷酸甘露糖可抑制人外周血白细胞的NK活性。然而，以下观察结果排除了6-磷酸甘露糖受体-配体系统在NK细胞介导的细胞毒性中的关键作用。1）NK细胞毒性对多种结构不相关的糖磷酸敏感。2）I细胞病（粘脂贮积症II）患者的NK活性正常，该病由于遗传缺陷而无法合成6-磷酸甘露糖特异性受体的配体。3）针对6-磷酸甘露糖受体的抗血清不会抑制NK细胞毒性，该抗血清会阻断受体功能。
