Rossi-Fanelli F, Cangiano C, Smith A R, Bozzi A, James J H, Kay L A, Perelle B A, Capocaccia L, Fischer J E
Ital J Biochem. 1978 Nov-Dec;27(6):450-65.
A sensitive radioenzymatic assay for the simultaneous determination of phenylethanolamine and octopamine in biological fluids is described. It is derived from the radioenzymatic assay originally described by Molinoff et al. (1969) and subsequently modified by Saavedra (1974). The enzymatic reaction is based upon the methylation of phenylethanolamine and octopamine by phenylethanolamine-N-methyl transferase using 14C-S-adenosylmethionine as the methyl donor. The N-methyl derivatives of the two amines are separately extracted and estimated. Selectivity is increased by optimization of extraction and evaporation and by subsequent extraction of the two compounds. Phenylethanolamine and octopamine levels were determined in plasma of human subjects and in plasma and CSF of dogs. The levels were found significantly elevated both in human and experimental hepatic encephalopathy.
本文描述了一种用于同时测定生物体液中苯乙醇胺和章鱼胺的灵敏放射酶分析法。它源自Molinoff等人（1969年）最初描述并随后由Saavedra（1974年）修改的放射酶分析法。酶促反应基于苯乙醇胺-N-甲基转移酶以14C-S-腺苷甲硫氨酸作为甲基供体对苯乙醇胺和章鱼胺进行甲基化。两种胺的N-甲基衍生物分别进行提取和测定。通过优化提取和蒸发以及随后对两种化合物的提取来提高选择性。测定了人类受试者血浆以及犬血浆和脑脊液中的苯乙醇胺和章鱼胺水平。发现在人类和实验性肝性脑病中这些水平均显著升高。
