Department of Evolutionary Zoology and Human Biology, University of Debrecen, Debrecen, Hungary.
HUN-REN-UD Behavioural Ecology Research Group, University of Debrecen, Debrecen, Hungary.
Sci Rep. 2024 Mar 30;14(1):7545. doi: 10.1038/s41598-024-58096-6.
Vector-borne diseases pose a potential risk to human and animal welfare, and understanding their spread requires genomic resources. The mosquito Aedes koreicus is an emerging vector that has been introduced into Europe more than 15 years ago but only a low quality, fragmented genome was available. In this study, we carried out additional sequencing and assembled and characterized the genome of the species to provide a background for understanding its evolution and biology. The updated genome was 1.1 Gbp long and consisted of 6099 contigs with an N50 value of 329,610 bp and a BUSCO score of 84%. We identified 22,580 genes that could be functionally annotated and paid particular attention to the identification of potential insecticide resistance genes. The assessment of the orthology of the genes indicates a high turnover at the terminal branches of the species tree of mosquitoes with complete genomes, which could contribute to the adaptation and evolutionary success of the species. These results could form the basis for numerous downstream analyzes to develop targets for the control of mosquito populations.
虫媒病对人类和动物的健康构成潜在威胁,而了解其传播方式需要基因组资源。白纹伊蚊是一种新兴的传播媒介,15 多年前就已传入欧洲,但当时仅有低质量、碎片化的基因组可供利用。在本研究中,我们进行了额外的测序,并组装和分析了该物种的基因组,为了解其进化和生物学特性提供了背景。更新后的基因组长 1.1Gbp,由 6099 个连续序列组成,N50 值为 329610bp,BUSCO 评分为 84%。我们鉴定了 22580 个具有功能注释的基因,并特别关注了潜在杀虫剂抗性基因的鉴定。基因的同源性评估表明,具有完整基因组的蚊子种系树的末端分支具有较高的周转率,这可能有助于该物种的适应和进化成功。这些结果可以为开发控制蚊子种群的目标提供众多下游分析的基础。