Laboratório de Pesquisa em Cirurgia Torácica, Departamento de Cardiopneumologia, Instituto do Coracao, Faculdade de Medicina HCFMUSP, Universidade de São Paulo.
Laboratório de Pesquisa em Cirurgia Torácica, Departamento de Cardiopneumologia, Instituto do Coracao, Faculdade de Medicina HCFMUSP, Universidade de São Paulo;
J Vis Exp. 2024 Mar 15(205). doi: 10.3791/62975.
Experimental models are important tools for understanding the etiological phenomena involved in various pathophysiological events. In this context, different animal models are used to study the elements triggering the pathophysiology of primary graft dysfunction after transplantation to evaluate potential treatments. Currently, we can divide experimental donation models into two large groups: donation after brain death and donation after circulatory arrest. In addition, the deleterious effects associated with hemorrhagic shock should be considered when considering animal models of organ donation. Here, we describe the establishment of three different lung donation models (post-brain death donation, post-circulatory death donation, and post-hemorrhagic shock donation) and compare the inflammatory processes and pathological disorders associated with these events. The objective is to provide the scientific community with reliable animal models of lung donation for studying the associated pathological mechanisms and searching for new therapeutic targets to optimize the number of viable grafts for transplantation.
实验模型是理解各种病理生理事件中病因现象的重要工具。在这种情况下，使用不同的动物模型来研究触发移植后原发性移植物功能障碍的病理生理学的因素，以评估潜在的治疗方法。目前，我们可以将实验性供体模型分为两大类：脑死亡后供体和心脏停搏后供体。此外，在考虑器官捐献的动物模型时，应考虑与出血性休克相关的有害影响。在这里，我们描述了三种不同的肺捐献模型（脑死亡后捐献、心脏停搏后捐献和出血性休克后捐献）的建立，并比较了这些事件相关的炎症过程和病理紊乱。目的是为科学界提供可靠的肺捐献动物模型，以研究相关的病理机制，并寻找新的治疗靶点，以优化用于移植的有活力的移植物数量。
