Department of Meat and Marine Sciences, CSIR-Central Food Technological Research Institute, Mysuru-570 020, Karnataka, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India.
Mar Biotechnol (NY). 2024 Apr;26(2):404-420. doi: 10.1007/s10126-024-10305-z. Epub 2024 Apr 1.
Optimization of antioxidants and angiotensin-converting enzyme (ACE) inhibitory potential gelatin hydrolysate production from Labeo rohita (rohu) swim bladder (SBGH) by alcalase using central composite design (CCD) of response surface methodology (RSM) was investigated. The maximum degree of hydrolysis (DH), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), total antioxidants (TAO), and ACE inhibitory activity were achieved at 0.1:1.0 (w/w) enzyme to substrate ratio, 61 °C hydrolysis temperature, and 94-min hydrolysis time. The resulting SBGH obtained at 19.92% DH exhibited the DPPH (24.28 µM TE/mg protein), ABTS (34.47 µM TE/mg protein), TAO (12.01 µg AAE/mg protein), and ACE inhibitory (4.91 µg/mg protein) activity. Furthermore, SBGH at 100 µg/ml displayed osteogenic property without any toxic effects on MC3T3-E1 cells. Besides, the protein content of rohu swim bladder gelatin (SBG) and SBGH was 93.68% and 94.98%, respectively. Both SBG and SBGH were rich in glycine, proline, glutamic acid, alanine, arginine, and hydroxyproline amino acids. Therefore, SBGH could be an effective nutraceutical in functional food development.
采用响应面法中的中心复合设计（CCD）优化罗非鱼鳔（SBGH）碱性蛋白酶水解制备抗氧化和血管紧张素转化酶（ACE）抑制肽的工艺条件。研究结果表明，当酶底物比为 0.1:1.0（w/w）、水解温度为 61°C、水解时间为 94min 时，水解度（DH）、2，2-二苯基-1-苦基肼基（DPPH）、2，2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸（ABTS）、总抗氧化能力（TAO）和 ACE 抑制活性均达到最大值。在 19.92%DH 条件下得到的 SBGH 的 DPPH（24.28µM TE/mg 蛋白）、ABTS（34.47µM TE/mg 蛋白）、TAO（12.01µg AAE/mg 蛋白）和 ACE 抑制活性（4.91µg/mg 蛋白）最高。此外，100µg/ml 的 SBGH 对 MC3T3-E1 细胞没有任何毒性作用，表现出成骨特性。另外，罗非鱼鳔明胶（SBG）和 SBGH 的蛋白质含量分别为 93.68%和 94.98%。SBG 和 SBGH 均富含甘氨酸、脯氨酸、谷氨酸、丙氨酸、精氨酸和羟脯氨酸。因此，SBGH 可以作为功能性食品开发的有效营养保健品。
