一种由与锌指蛋白融合的多功能嵌合肽组成的非病毒DNA递送系统。

A non-viral DNA delivery system consisting of multifunctional chimeric peptide fused with zinc-finger protein.

作者信息

Yu Siyuan, Pan Haifeng, Yang Han, Zhuang Haoyun, Yang Haihui, Yu Xuan, Zhang Shiyin, Fang Mujin, Li Tingdong, Ge Shengxiang, Xia Ningshao

机构信息

State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, Department of Laboratory Medicine, School of Public Health, Xiamen University, Xiamen 361102, China.

National Institute of Diagnostics and Vaccine Development in Infectious Diseases, State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Collaborative Innovation Center of Biologic Products, National Innovation Platform for Industry-Education Integration in Vaccine Research, NMPA Key Laboratory for Research and Evaluation of Infectious Disease Diagnostic Technology, the Research Unit of Frontier Technology of Structural Vaccinology of Chinese Academy of Medical Sciences, Xiamen University, Xiamen 361102, China.

出版信息

iScience. 2024 Mar 8;27(4):109464. doi: 10.1016/j.isci.2024.109464. eCollection 2024 Apr 19.

DOI:10.1016/j.isci.2024.109464

PMID:38558940

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10981093/

Abstract

Non-viral gene delivery systems have received sustained attention as a promising alternative to viral vectors for disease treatment and prevention in recent years. Numerous methods have been developed to enhance gene uptake and delivery in the cytoplasm; however, due to technical difficulties and delivery efficiency, these systems still face challenges in a range of biological applications, especially . To alleviate this challenge, we devised a novel system for gene delivery based on a recombinant protein eTAT-ZF9-NLS, which consisted of a multifunctional chimeric peptide and a zinc-finger protein with sequence-specific DNA-binding activity. High transfection efficiency was observed in several mammalian cells after intracellular delivery of plasmid containing ZF9-binding sites mediated by eTAT-ZF9-NLS. Our new approach provides a novel transfection strategy and the transfection efficiency was confirmed both and , making it a preferential transfection reagent for possible gene therapy.

摘要

近年来，非病毒基因递送系统作为一种有前景的替代病毒载体用于疾病治疗和预防的方法，受到了持续关注。人们已经开发出许多方法来增强基因在细胞质中的摄取和递送；然而，由于技术困难和递送效率问题，这些系统在一系列生物学应用中仍然面临挑战，尤其是……为了缓解这一挑战，我们设计了一种基于重组蛋白eTAT-ZF9-NLS的新型基因递送系统，该系统由一个多功能嵌合肽和一个具有序列特异性DNA结合活性的锌指蛋白组成。在由eTAT-ZF9-NLS介导的含有ZF9结合位点的质粒细胞内递送后，在几种哺乳动物细胞中观察到了高转染效率。我们的新方法提供了一种新颖的转染策略，并且转染效率在……和……中均得到了证实，使其成为可能的基因治疗的优先转染试剂。