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模式昆虫的肠道超微结构表面图谱

An enteric ultrastructural surface atlas of the model insect .

作者信息

Windfelder Anton G, Steinbart Jessica, Graser Leonie, Scherberich Jan, Krombach Gabriele A, Vilcinskas Andreas

机构信息

Branch for Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Giessen, Germany.

Experimental Radiology, Department of Diagnostic and Interventional Radiology, University-Hospital Giessen, Justus Liebig University Giessen, Giessen, Germany.

出版信息

iScience. 2024 Mar 4;27(4):109410. doi: 10.1016/j.isci.2024.109410. eCollection 2024 Apr 19.

DOI:10.1016/j.isci.2024.109410
PMID:38558941
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10981077/
Abstract

The tobacco hornworm is a laboratory model that is particularly suitable for analyzing gut inflammation, but a physiological reference standard is currently unavailable. Here, we present a surface atlas of the healthy hornworm gut generated by scanning electron microscopy and nano-computed tomography. This comprehensive overview of the gut surface reveals morphological differences between the anterior, middle, and posterior midgut, allowing the screening of aberrant gut phenotypes while accommodating normal physiological variations. We estimated a total resorptive midgut surface of 0.42 m for L5d6 larvae, revealing its remarkable size. Our data will support allometric scaling and dose conversion from to mammals in preclinical research, embracing the 3R principles. We also observed non-uniform gut colonization by enterococci, characterized by dense biofilms in the pyloric cone and downstream of the pylorus associated with pore and spine structures in the hindgut intima, indicating a putative immunosurveillance function in the lepidopteran hindgut.

摘要

烟草天蛾是一种特别适合用于分析肠道炎症的实验室模型，但目前尚无生理参考标准。在此，我们展示了通过扫描电子显微镜和纳米计算机断层扫描生成的健康烟草天蛾肠道表面图谱。对肠道表面的这一全面概述揭示了中肠前部、中部和后部之间的形态差异，在适应正常生理变异的同时，有助于筛选异常肠道表型。我们估计，L5d6幼虫的中肠总吸收表面积为0.42平方米，显示出其显著的大小。我们的数据将支持临床前研究中从昆虫到哺乳动物的异速生长缩放和剂量转换，遵循3R原则。我们还观察到肠球菌在肠道中的定殖并不均匀，其特征是幽门锥和幽门下游存在密集的生物膜，这些生物膜与后肠内膜中的孔和棘结构相关，表明鳞翅目昆虫后肠具有假定的免疫监视功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abda/10981077/1032d620fd8c/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abda/10981077/31c592a55a9d/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abda/10981077/4321b3884852/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abda/10981077/7d107ca63c4a/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abda/10981077/b786a3451ea5/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abda/10981077/912282657a4b/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abda/10981077/ea97738311cc/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abda/10981077/378b2eae26da/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abda/10981077/7ce67af1e631/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abda/10981077/09e178b8ff92/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abda/10981077/73daa045fa00/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abda/10981077/0c8e005ad44f/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abda/10981077/1032d620fd8c/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abda/10981077/31c592a55a9d/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abda/10981077/4321b3884852/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abda/10981077/7d107ca63c4a/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abda/10981077/b786a3451ea5/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abda/10981077/912282657a4b/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abda/10981077/ea97738311cc/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abda/10981077/378b2eae26da/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abda/10981077/7ce67af1e631/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abda/10981077/09e178b8ff92/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abda/10981077/73daa045fa00/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abda/10981077/0c8e005ad44f/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/abda/10981077/1032d620fd8c/gr11.jpg

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本文引用的文献

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J Clin Med. 2023 Dec 15;12(24):7718. doi: 10.3390/jcm12247718.
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Dextran sulfate sodium and uracil induce inflammatory effects and disrupt the chitinous peritrophic matrix in the midgut of Tribolium castaneum.硫酸葡聚糖钠和尿嘧啶会引发炎症反应，并破坏赤拟谷盗中肠的几丁质围食膜。
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Differential expression of immunity-related genes in larval tissues in response to gut and systemic infection.
免疫相关基因在幼虫组织中对肠道和系统感染的差异表达。
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Fast enterocyte regrowth after infection involves a reverse metabolic flux driven by an amino acid transporter.感染后快速的肠上皮细胞再生涉及由一种氨基酸转运体驱动的逆向代谢通量。
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Powerful and Real-Time Quantification of Antifungal Efficacy against Triazole-Resistant and -Susceptible Aspergillus fumigatus Infections in Galleria mellonella by Longitudinal Bioluminescence Imaging.利用纵向生物发光成像技术对烟曲霉感染进行强大且实时的抗真菌疗效定量评估：耐唑类和敏感烟曲霉。
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