Department of Social and Applied Science, College of Industrial Technology, King Mongkut's University of Technology North Bangkok, Bangkok, Thailand.
Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
PLoS One. 2024 Apr 2;19(4):e0299987. doi: 10.1371/journal.pone.0299987. eCollection 2024.
This study aimed to investigate AMR profiles of Aeromonas hydrophila, Salmonella spp., and Vibrio cholerae isolated from Nile tilapia (Oreochromis spp.) (n = 276) purchased from fresh markets and supermarkets in Bangkok, Thailand. A sample of tilapia was divided into three parts: fish intestine (n = 276), fish meat (n = 276), and liver and kidney (n = 276). The occurrence of A. hydrophila, Salmonella, and V. cholerae was 3.1%, 7.4%, and 8.5%, respectively. A high prevalence of these pathogenic bacteria was observed in fresh market tilapia compared to those from supermarkets (p < 0.05). The predominant Salmonella serovars were Paratyphi B (6.4%), followed by Escanaba (5.7%), and Saintpaul (5.7%). All isolates tested positive for the virulence genes of A. hydrophila (aero and hly), Salmonella (invA), and V. cholerae (hlyA). A. hydrophila (65.4%), Salmonella (31.2%), and V. cholerae (2.9%) showed multidrug resistant isolates. All A. hydrophila isolates (n = 26) exhibited resistant to ampicillin (100.0%) and florfenicol (100.0%), and often carried sul1 (53.8%) and tetA (50.0%). Salmonella isolates were primarily resistant to ampicillin (36.9%), with a high incidence of blaTEM (26.2%) and qnrS (25.5%). For V. cholerae isolates, resistance was observed against ampicillin (48.6%), and they commonly carried qnrS (24.3%) and tetA (22.9%). To identify mutations in the quinolone resistance determining regions (QRDRs), a single C248A point mutation of C248A (Ser-83-Tyr) in the gyrA region was identified in six out of seven isolates of Salmonella isolates. This study highlighted the presence of antimicrobial-resistant pathogenic bacteria in Nile tilapia at a selling point. It is important to rigorously implement strategies for AMR control and prevention.
本研究旨在调查从泰国曼谷的新鲜市场和超市购买的尼罗罗非鱼（Oreochromis spp.）（n = 276）中分离的嗜水气单胞菌、沙门氏菌和霍乱弧菌的 AMR 谱。罗非鱼样本分为三部分：鱼肠（n = 276）、鱼肉（n = 276）和肝、肾（n = 276）。嗜水气单胞菌、沙门氏菌和霍乱弧菌的发生率分别为 3.1%、7.4%和 8.5%。与超市相比，新鲜市场罗非鱼中这些致病菌的高发率更高（p < 0.05）。主要的沙门氏菌血清型为副伤寒 B（6.4%），其次是埃斯卡诺巴（5.7%）和圣保禄（5.7%）。所有分离株均检测到嗜水气单胞菌（aero 和 hly）、沙门氏菌（invA）和霍乱弧菌（hlyA）的毒力基因阳性。嗜水气单胞菌（65.4%）、沙门氏菌（31.2%）和霍乱弧菌（2.9%）表现为多药耐药株。所有嗜水气单胞菌分离株（n = 26）均对氨苄西林（100.0%）和氟苯尼考（100.0%）表现出耐药性，且常常携带 sul1（53.8%）和 tetA（50.0%）。沙门氏菌分离株主要对氨苄西林（36.9%）耐药，blaTEM（26.2%）和 qnrS（25.5%）的发生率较高。对于霍乱弧菌分离株，对氨苄西林（48.6%）表现出耐药性，它们通常携带 qnrS（24.3%）和 tetA（22.9%）。为了鉴定喹诺酮类药物耐药决定区（QRDRs）中的突变，在 7 株沙门氏菌分离株中有 6 株发现了 gyrA 区单个 C248A 点突变 C248A（Ser-83-Tyr）。本研究强调了在销售点存在携带抗微生物药物耐药性的致病性细菌的情况。严格执行抗微生物药物耐药性控制和预防策略非常重要。
