Reed J A, Kough R H, Williamson P L, Schlegel R A
Cell Biol Int Rep. 1985 Jan;9(1):43-9. doi: 10.1016/0309-1651(85)90140-7.
Merocyanine 540 is a fluorescent dye which stains erythrocytes that have lost their normal membrane phospholipid asymmetry. Because erythrocytes from patients with chronic myelogenous leukemia have been reported to display this abnormal phenotype, peripheral blood erythrocytes from such patients were examined for their ability to stain with the dye. Erythrocytes from all patients with active disease states stained, whereas neither erythrocytes from normal, healthy individuals nor from a patient whose disease symptoms were eliminated by chemotherapy stained. These results suggest that merocyanine 540 may have utility in the clinical evaluation of chronic myelogenous leukemia.
部花青540是一种荧光染料，可对失去正常膜磷脂不对称性的红细胞进行染色。由于据报道慢性粒细胞白血病患者的红细胞会呈现这种异常表型，因此对这类患者的外周血红细胞进行了该染料染色能力的检测。所有处于疾病活动期患者的红细胞都被染色，而正常健康个体的红细胞以及疾病症状通过化疗消除的患者的红细胞均未被染色。这些结果表明，部花青540可能在慢性粒细胞白血病的临床评估中具有应用价值。
