• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

人类RhD和RhAG血型蛋白的分子动力学

Molecular dynamics of the human RhD and RhAG blood group proteins.

作者信息

Floch Aline, Galochkina Tatiana, Pirenne France, Tournamille Christophe, de Brevern Alexandre G

机构信息

University Paris Est Créteil, INSERM U955 Equipe Transfusion et Maladies du Globule Rouge, IMRB, Créteil, France.

Laboratoire de Biologie Médicale de Référence en Immuno-Hématologie Moléculaire, Etablissement Français du Sang Ile-de-France, Créteil, France.

出版信息

Front Chem. 2024 Mar 19;12:1360392. doi: 10.3389/fchem.2024.1360392. eCollection 2024.

DOI:10.3389/fchem.2024.1360392
PMID:38566898
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10985258/
Abstract

Blood group antigens of the RH system (formerly known as "Rhesus") play an important role in transfusion medicine because of the severe haemolytic consequences of antibodies to these antigens. No crystal structure is available for RhD proteins with its partner RhAG, and the precise stoichiometry of the trimer complex remains unknown. To analyse their structural properties, the trimers formed by RhD and/or RhAG subunits were generated by protein modelling and molecular dynamics simulations were performed. No major differences in structural behaviour were found between trimers of different compositions. The conformation of the subunits is relatively constant during molecular dynamics simulations, except for three large disordered loops. This work makes it possible to propose a reasonable stoichiometry and demonstrates the potential of studying the structural behaviour of these proteins to investigate the hundreds of genetic variants relevant to transfusion medicine.

摘要

RH系统（以前称为“恒河猴”）的血型抗原在输血医学中起着重要作用，因为针对这些抗原的抗体具有严重的溶血后果。目前尚无RhD蛋白与其伴侣RhAG的晶体结构，三聚体复合物的确切化学计量仍不清楚。为了分析它们的结构特性，通过蛋白质建模生成了由RhD和/或RhAG亚基形成的三聚体，并进行了分子动力学模拟。不同组成的三聚体在结构行为上未发现重大差异。除了三个大的无序环外，亚基的构象在分子动力学模拟过程中相对恒定。这项工作使得提出合理的化学计量成为可能，并证明了研究这些蛋白质的结构行为以研究与输血医学相关的数百种基因变体的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6af/10985258/b754f83ee66e/fchem-12-1360392-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6af/10985258/7e89e2b7052e/fchem-12-1360392-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6af/10985258/e20b945ff0d9/fchem-12-1360392-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6af/10985258/ac4a285189ab/fchem-12-1360392-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6af/10985258/43011b0b590d/fchem-12-1360392-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6af/10985258/b2a5b4d356dc/fchem-12-1360392-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6af/10985258/b754f83ee66e/fchem-12-1360392-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6af/10985258/7e89e2b7052e/fchem-12-1360392-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6af/10985258/e20b945ff0d9/fchem-12-1360392-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6af/10985258/ac4a285189ab/fchem-12-1360392-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6af/10985258/43011b0b590d/fchem-12-1360392-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6af/10985258/b2a5b4d356dc/fchem-12-1360392-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f6af/10985258/b754f83ee66e/fchem-12-1360392-g006.jpg

相似文献

1
Molecular dynamics of the human RhD and RhAG blood group proteins.人类RhD和RhAG血型蛋白的分子动力学
Front Chem. 2024 Mar 19;12:1360392. doi: 10.3389/fchem.2024.1360392. eCollection 2024.
2
Cell-surface expression of RhD blood group polypeptide is posttranscriptionally regulated by the RhAG glycoprotein.RhD血型多肽的细胞表面表达受RhAG糖蛋白的转录后调控。
Blood. 2002 Aug 1;100(3):1038-47.
3
Mice expressing RHAG and RHD human blood group genes.表达RHAG和RHD人类血型基因的小鼠。
PLoS One. 2013 Nov 18;8(11):e80460. doi: 10.1371/journal.pone.0080460. eCollection 2013.
4
The human Rhesus-associated RhAG protein and a kidney homologue promote ammonium transport in yeast.人类恒河猴相关RhAG蛋白及其肾脏同源物促进酵母中的铵转运。
Nat Genet. 2000 Nov;26(3):341-4. doi: 10.1038/81656.
5
A variant RhAG protein encoded by the RHAG*572A allele causes serological weak D expression while maintaining normal RhCE phenotypes.由RHAG*572A等位基因编码的一种变异RhAG蛋白导致血清学弱D表达，同时保持正常的RhCE表型。
Transfusion. 2019 Jan;59(1):405-411. doi: 10.1111/trf.14969. Epub 2018 Oct 4.
6
Generation and characterisation of Rhd and Rhag null mice.Rhd 和 Rhag 基因缺失小鼠的构建及鉴定。
Br J Haematol. 2010 Jan;148(1):161-72. doi: 10.1111/j.1365-2141.2009.07928.x. Epub 2009 Oct 5.
7
Molecular biology of Rh proteins and relevance to molecular medicine.Rh蛋白的分子生物学及其与分子医学的相关性。
Expert Rev Mol Med. 2006 Jun 12;8(13):1-20. doi: 10.1017/S1462399406010969.
8
A RHAG point mutation selectively disrupts Rh antigen expression.一个RHAG点突变选择性地破坏Rh抗原表达。
Transfus Med. 2019 Apr;29(2):121-127. doi: 10.1111/tme.12519. Epub 2018 Mar 6.
9
Compound heterozygosity of two novel RHAG alleles leads to a considerable disruption of the Rh complex.两个新型RHAG等位基因的复合杂合性导致Rh复合体出现相当大的破坏。
Transfusion. 2016 Apr;56(4):950-5. doi: 10.1111/trf.13476.
10
Rh proteins: key structural and functional components of the red cell membrane.Rh蛋白：红细胞膜的关键结构和功能成分。
Blood Rev. 2006 Mar;20(2):93-110. doi: 10.1016/j.blre.2005.04.002. Epub 2005 Jun 14.

引用本文的文献

1
Integrating Genotyping for More Accurate Rh(D) Antigen Phenotyping: A Retrospective Study.整合基因分型以实现更准确的Rh(D)抗原表型分析：一项回顾性研究
Medicina (Kaunas). 2025 Apr 5;61(4):670. doi: 10.3390/medicina61040670.

本文引用的文献

1
How accurately can one predict drug binding modes using AlphaFold models?使用 AlphaFold 模型能多准确地预测药物结合模式？
Elife. 2023 Dec 22;12:RP89386. doi: 10.7554/eLife.89386.
2
Use of computational biology to compare the theoretical tertiary structures of the most common forms of RhCE and RhD.运用计算生物学比较RhCE和RhD最常见形式的理论三级结构。
Vox Sang. 2023 Oct;118(10):881-890. doi: 10.1111/vox.13509. Epub 2023 Aug 9.
3
A novel high-prevalence antigen in the Lutheran system, LUGA (LU24), and an updated, full-length 3D BCAM model.
路德系统中一种新型高流行抗原LUGA（LU24）以及更新的全长三维基底细胞黏附分子模型。
Transfusion. 2023 Apr;63(4):798-807. doi: 10.1111/trf.17262. Epub 2023 Feb 4.
4
A Structure-Based View on ABC-Transporter Linked to Multidrug Resistance.基于结构的观点看 ABC 转运蛋白与多药耐药的关系。
Molecules. 2023 Jan 4;28(2):495. doi: 10.3390/molecules28020495.
5
Two new Scianna variants causing loss of high prevalence antigens: ERMAP model and 3D analysis of the antigens.两种新的 Scianna 变异体导致高流行抗原丢失：ERMAP 模型和抗原的 3D 分析。
Transfusion. 2023 Jan;63(1):230-238. doi: 10.1111/trf.17182. Epub 2022 Nov 8.
6
TmAlphaFold database: membrane localization and evaluation of AlphaFold2 predicted alpha-helical transmembrane protein structures.TmAlphaFold 数据库：AlphaFold2 预测的 α-螺旋跨膜蛋白结构的膜定位和评估。
Nucleic Acids Res. 2023 Jan 6;51(D1):D517-D522. doi: 10.1093/nar/gkac928.
7
Editorial: Combined artificial intelligence and molecular dynamics (AI-MD) methods.社论：人工智能与分子动力学相结合的方法
Front Mol Biosci. 2022 Oct 6;9:1012785. doi: 10.3389/fmolb.2022.1012785. eCollection 2022.
8
Architecture of the human erythrocyte ankyrin-1 complex.人类红细胞锚蛋白-1 复合物的结构。
Nat Struct Mol Biol. 2022 Jul;29(7):706-718. doi: 10.1038/s41594-022-00792-w. Epub 2022 Jul 14.
9
AlphaFold2 versus experimental structures: evaluation on G protein-coupled receptors.AlphaFold2 与实验结构的比较：在 G 蛋白偶联受体上的评估。
Acta Pharmacol Sin. 2023 Jan;44(1):1-7. doi: 10.1038/s41401-022-00938-y. Epub 2022 Jul 1.
10
Improving the topology prediction of α-helical transmembrane proteins with deep transfer learning.利用深度迁移学习改进α-螺旋跨膜蛋白的拓扑结构预测
Comput Struct Biotechnol J. 2022 Apr 20;20:1993-2000. doi: 10.1016/j.csbj.2022.04.024. eCollection 2022.