da Rosa Larissa C, Scales Hannah E, Makhija Sangeet, Sutherland Katie, Benson Robert A, Brewer James M, Garside Paul
School of Infection and Immunity, College of Medical, Veterinary, and Life Sciences, University of Glasgow, Glasgow G12 8TA, UK.
Discov Immunol. 2022 Nov 21;1(1):kyac008. doi: 10.1093/discim/kyac008. eCollection 2022.
One of the earliest signs of dysregulation of the homeostatic process of fibrosis, associated with pathology in chronic conditions such as rheumatoid arthritis, is the overexpression of collagen type III (COL-3). Critically, there is still relatively little known regarding the identity of the cell types expressing the gene encoding COL-3 (). Identifying and characterizing -expressing cells during the development of fibrosis could reveal new targets for the diagnosis and treatment of fibrosis-related pathologies. As such, a reporter mouse expressing concomitantly and mKate-2, a fluorescent protein, was generated. Using models of footpad inflammation, we demonstrated its effectiveness as a tool to measure the expression of COL-3 during the repair process and provided an initial characterization of some of the stromal and immune cells responsible for expression.
与类风湿关节炎等慢性疾病的病理状况相关的纤维化稳态过程失调的最早迹象之一是III型胶原蛋白（COL-3）的过度表达。至关重要的是，关于表达编码COL-3基因的细胞类型的身份仍然知之甚少。在纤维化发展过程中识别和表征表达COL-3的细胞可能会揭示纤维化相关病理诊断和治疗的新靶点。因此，构建了一种同时表达COL-3和荧光蛋白mKate-2的报告小鼠。利用足垫炎症模型，我们证明了它作为一种工具在修复过程中测量COL-3表达的有效性，并对一些负责COL-3表达的基质细胞和免疫细胞进行了初步表征。
