用3'O-(4-苯甲酰基)苯甲酰腺苷5'-三磷酸对叶绿体偶联因子1上核苷酸结合位点的研究。

Investigation of nucleotide binding sites on chloroplast coupling factor 1 with 3'O-(4-benzoyl)benzoyl adenosine 5'-triphosphate.

作者信息

Kambouris N G, Hammes G G

出版信息

Proc Natl Acad Sci U S A. 1985 Apr;82(7):1950-3. doi: 10.1073/pnas.82.7.1950.

DOI:10.1073/pnas.82.7.1950

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC397456/

Abstract

The subunit locations of each of the three nucleotide binding sites of soluble chloroplast coupling factor 1 have been studied with the photoaffinity label 3'-O-(4-benzoyl)benzoyl-ATP. This derivative is an effective inhibitor of ATPase activity. Photolysis of the radioactive label when bound to each of the three nucleotide sites on the coupling factor has been examined. For the nucleotide site that normally binds ADP very tightly, NaDodSO4/polyacrylamide gel electrophoresis after photolysis indicates that primarily the beta polypeptide chain is appreciably labeled (86%), although some labeling of the alpha polypeptide chain is found (14%). For the site that binds MgATP tightly, 97% of the radioactivity is found on the beta polypeptide chain. The alpha and beta polypeptide chains are labeled in approximately equal amounts when photolysis is carried out with the nucleotide analog bound to the third site.

摘要

利用光亲和标记物3'-O-(4-苯甲酰基)苯甲酰基-ATP研究了可溶性叶绿体偶联因子1的三个核苷酸结合位点中每个位点的亚基位置。该衍生物是ATP酶活性的有效抑制剂。已检测了放射性标记物与偶联因子上三个核苷酸位点中的每一个位点结合时的光解情况。对于通常紧密结合ADP的核苷酸位点，光解后的十二烷基硫酸钠/聚丙烯酰胺凝胶电泳表明，主要是β多肽链被显著标记（86%），尽管也发现了一些α多肽链的标记（14%）。对于紧密结合MgATP的位点，97%的放射性出现在β多肽链上。当用与第三个位点结合的核苷酸类似物进行光解时，α和β多肽链的标记量大致相等。

相似文献

1

Investigation of nucleotide binding sites on chloroplast coupling factor 1 with 3'O-(4-benzoyl)benzoyl adenosine 5'-triphosphate.用3'O-(4-苯甲酰基)苯甲酰腺苷5'-三磷酸对叶绿体偶联因子1上核苷酸结合位点的研究。

Proc Natl Acad Sci U S A. 1985 Apr;82(7):1950-3. doi: 10.1073/pnas.82.7.1950.

2

Amino acid sequence of the nucleotide binding region of chloroplast coupling factor 1.

Biochemistry. 1987 Jun 2;26(11):3193-7. doi: 10.1021/bi00385a038.

3

Localization of the high-affinity binding site for ATP on the membrane-bound chloroplast ATP synthase.ATP在膜结合叶绿体ATP合酶上高亲和力结合位点的定位。

J Biol Chem. 1984 Oct 10;259(19):12271-8.

4

Modification of domains of alpha and beta subunits of F1-ATPase from the thermophylic bacterium PS3, in their isolated and associated forms, by 3'-O-(4-benzoyl)benzoyl adenosine 5'-triphosphate (BzATP).嗜热细菌PS3的F1-ATP酶α和β亚基结构域在其分离形式和结合形式下被3'-O-(4-苯甲酰基)苯甲酰腺苷5'-三磷酸（BzATP）修饰。

J Bioenerg Biomembr. 1996 Dec;28(6):471-81. doi: 10.1007/BF02110437.

5

The AMP-binding domain on adenylate kinase. Evidence for a conformational change during binary-to-ternary complex formation via photoaffinity labeling analyses.腺苷酸激酶上的AMP结合结构域。通过光亲和标记分析在二元复合物到三元复合物形成过程中构象变化的证据。

J Biol Chem. 1992 Dec 15;267(35):25003-9.

6

Identification of 3-O-(4-benzoyl)benzoyladenosine 5'-triphosphate- and N,N'-dicyclohexylcarbodiimide-binding subunits of a higher plant H+-translocating tonoplast ATPase.高等植物液泡膜H⁺转运ATP酶的3 - O -（4 - 苯甲酰基）苯甲酰腺苷5'-三磷酸和N，N'-二环己基碳二亚胺结合亚基的鉴定

J Biol Chem. 1985 Oct 5;260(22):12273-9.

7

Labeling of Chlamydomonas 18 S dynein polypeptides by 8-azidoadenosine 5'-triphosphate, a photoaffinity analog of ATP.利用ATP的光亲和类似物8-叠氮腺苷5'-三磷酸对衣藻18S动力蛋白多肽进行标记。

J Biol Chem. 1985 Oct 15;260(23):12844-50.

8

Localization of the ATP binding site on alpha-tubulin.α-微管蛋白上ATP结合位点的定位

Arch Biochem Biophys. 1983 Sep;225(2):475-81. doi: 10.1016/0003-9861(83)90056-5.

9

8-Azido-adenosine 5'-triphosphate as a photoaffinity label for bacterial F1 ATPase.

Eur J Biochem. 1978 Jul 17;88(1):253-7. doi: 10.1111/j.1432-1033.1978.tb12445.x.

10

Identification of the catalytic subunit of an oligomeric casein kinase (G type). Affinity labeling of the nucleotide site using 5'-[p-(fluorosulfonyl)benzoyl]adenosine.

Biochemistry. 1983 Mar 15;22(6):1452-9. doi: 10.1021/bi00275a020.

引用本文的文献

1

Activation of early events of the mitogenic response by a P2Y purinoceptor with covalently bound 3'-O-(4-benzoyl)-benzoyladenosine 5'-triphosphate.通过与共价结合的3'-O-(4-苯甲酰基)-苯甲酰腺苷5'-三磷酸的P2Y嘌呤受体激活有丝分裂原反应的早期事件。

Proc Natl Acad Sci U S A. 1990 Dec;87(24):9717-21. doi: 10.1073/pnas.87.24.9717.

本文引用的文献

1

Photoaffinity labeling with 2-azidoadenosine diphosphate of a tight nucleotide binding site on chloroplast coupling factor 1.用 2-叠氮腺苷二磷酸对叶绿体偶联因子 1 上的一个紧密核苷酸结合位点进行光亲和标记。

Proc Natl Acad Sci U S A. 1982 Dec;79(24):7744-8. doi: 10.1073/pnas.79.24.7744.

2

PARTIAL RESOLUTION OF THE ENZYMES CATALYZINE PHOTOPHOSPHORYLATION. I. STIMULATION OF PHOTOPHOSPHORYLATION BY A PREPARATION OF A LATENT, CA++- DEPENDENT ADENOSINE TRIPHOSPHATASE FROM CHLOROPLASTS.催化光磷酸化酶的部分分解。I. 叶绿体中一种潜在的、依赖Ca++的腺苷三磷酸酶制剂对光磷酸化的刺激作用。

J Biol Chem. 1965 Jun;240:2660-7.

3

Exploring the adenine nucleotide binding sites on mitochondrial F1-ATPase with a new photoaffinity probe, 3'-O-(4-benzoyl)benzoyl adenosine 5'-triphosphate.用新型光亲和探针3'-O-(4-苯甲酰基)苯甲酰腺苷5'-三磷酸探索线粒体F1-ATP酶上的腺嘌呤核苷酸结合位点。

J Biol Chem. 1982 Mar 25;257(6):2834-41.

4

Further characterization of nucleotide binding sites on chloroplast coupling factor one.叶绿体偶联因子1上核苷酸结合位点的进一步表征

Biochemistry. 1981 Oct 27;20(22):6298-305. doi: 10.1021/bi00525a003.

5

Energy transduction in chloroplasts: structure and function of the ATPase complex.叶绿体中的能量转换：ATP 酶复合体的结构与功能

Annu Rev Biochem. 1980;49:111-38. doi: 10.1146/annurev.bi.49.070180.000551.

6

Affinity labeling of purine nucleotide sites in proteins.蛋白质中嘌呤核苷酸位点的亲和标记

Annu Rev Biochem. 1983;52:67-91. doi: 10.1146/annurev.bi.52.070183.000435.

7

Probes of catalytic site cooperativity during catalysis by the chloroplast adenosine triphosphate and the adenosine triphosphate synthase.叶绿体三磷酸腺苷及三磷酸腺苷合酶催化过程中催化位点协同性的探究

J Biol Chem. 1983 Sep 25;258(18):10881-6.

8

Catalytic site cooperativity of beef heart mitochondrial F1 adenosine triphosphatase. Correlations of initial velocity, bound intermediate, and oxygen exchange measurements with an alternating three-site model.牛心线粒体F1三磷酸腺苷酶的催化位点协同性。初始速度、结合中间体及氧交换测量值与交替三位点模型的相关性。

J Biol Chem. 1982 Oct 25;257(20):12030-8.

9

Mechanism for catalysis and regulation of adenosine 5'-triphosphate hydrolysis by chloroplast coupling factor 1.叶绿体偶联因子1催化和调节腺苷5'-三磷酸水解的机制。

Biochemistry. 1982 Jul 6;21(14):3370-7. doi: 10.1021/bi00257a019.

10

Characterization of nucleotide binding sites on chloroplast coupling factor 1.叶绿体偶联因子1上核苷酸结合位点的特性分析

Biochemistry. 1975 Jul;14(13):2968-75. doi: 10.1021/bi00684a027.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。