School of Agriculture and Environment and the UWA Institute of Agriculture, The University of Western Australia, Crawley, WA 6009, Australia.
Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad 38000, Pakistan.
Plant Dis. 2024 Aug;108(8):2542-2549. doi: 10.1094/PDIS-03-24-0506-RE. Epub 2024 Aug 6.
Stem rot caused by is a serious and sometimes devastating disease of lupin ( spp.). A total of 236 lupin accessions from across 12 species were screened against the prevalent isolate MBRS-1 (pathotype 76). accession 21655 and var. accession 20589 showed immune and "near-immune" responses, respectively. Thirteen accessions of , three accessions each of and var. , and a single accession each of var. , , , and (totaling ∼4%) showed a highly resistant (HR) response. A further 19 accessions of , 2 accessions each of and , and a single accession of (totaling ∼10%) showed a resistant (R) response. The reactions of 16 (15 , 1 ) of these 236 accessions were also compared with their reactions to a different isolate, Walkaway-3 (WW-3; pathotype 10). Against this isolate, five accessions showed an HR response and four showed an R response, and the accession showed a moderate resistance response. Overall, isolate WW-3 caused significantly ( 0.05) smaller lesions than MBRS-1 across tested accessions in common. In addition, 328 plants in a "wild" naturalized field population of were screened in situ in the field against isolate MBRS-1. Five (∼1.5%) of the 328 plants of wild lupin showed an immune response, 63 (∼19%) showed an HR response, and 146 (∼45%) showed an R response. We believe this is the first examination of diverse spp. germplasm responses to a prevalent pathotype of . Lupin genotypes exhibiting high-level resistance to Sclerotinia stem rot identified in this study can be used as parental lines for crosses in lupin breeding programs and/or directly as improved cultivars to reduce the adverse impact of this disease on lupin crops.
由 引起的茎腐病是羽扇豆属（Lupinus）植物的一种严重且有时是毁灭性疾病。对来自 12 个种的 236 份羽扇豆种质资源进行了筛选，以鉴定对流行的 isolate MBRS-1（致病型 76）的抗性。accession 21655 和 var. accession 20589 分别表现出免疫和“近免疫”反应。 种的 13 个品系、 var. 的 3 个品系和 var. 、 var. 、 var. 、 和 的单个品系（总计约 4%）表现出高抗（HR）反应。 var. 的 19 个品系、 和 var. 的 2 个品系和 var. 的单个品系（总计约 10%）表现出抗（R）反应。对这 236 个品系中的 16 个品系（15 个 var. 、1 个 ）的反应也与它们对另一个分离株 Walkaway-3（WW-3；致病型 10）的反应进行了比较。对该分离株， var. 的 5 个品系表现出 HR 反应，4 个品系表现出 R 反应，而 var. 的 1 个品系表现出中度抗性反应。总体而言，与测试的共有品系相比，分离株 WW-3 引起的病变明显（ 0.05）更小。此外，对 自然归化野外种群中的 328 株植物进行了现场原位筛选，以鉴定对分离株 MBRS-1 的抗性。野生羽扇豆的 5 株（约 1.5%）表现出免疫反应，63 株（约 19%）表现出 HR 反应，146 株（约 45%）表现出 R 反应。我们相信，这是首次对不同的 spp. 种质资源对流行致病型的反应进行检查。本研究中鉴定出的对茎腐病具有高水平抗性的羽扇豆基因型可用于羽扇豆育种计划中的杂交亲本系，或直接作为改良品种，以减少该病害对羽扇豆作物的不利影响。
