Milleding P, Wennberg A, Hasselgren G
Scand J Dent Res. 1985 Feb;93(1):76-83. doi: 10.1111/j.1600-0722.1985.tb01312.x.
The cytotoxicity of one conventional and four non-gamma 2-amalgams was studied in a cell culture system, using the Millipore filter method. Before testing set amalgam specimens were kept in distilled water or in artificial saliva at pH 4, 5, or 7 for up to 28 wk to produce a corrosion layer on the test surface. Non-corroded set amalgam specimens was also tested. None of the noncorroded, set amalgams showed any sign of surface accumulation of cytotoxic products whereas the corroded amalgams showed varying degrees of cytotoxicity. Generally, the non-gamma 2-amalgams gave a more pronounced cytotoxic effect than the conventional amalgam. When the corrosion procedure was carried out at pH 7, the various non-gamma 2-amalgams showed different degrees of cytotoxicity. It appears that the difference in cytotoxic effect between the non-gamma 2-amalgams and the conventional amalgam as well as the differences among the various non-gamma 2-amalgams could be related to variation in the retention of corrosion products deposited on the amalgam surface.
采用密理博滤膜法，在细胞培养系统中研究了一种传统汞合金和四种不含γ2相汞合金的细胞毒性。在测试前，将凝固的汞合金试样置于蒸馏水或pH值为4、5或7的人工唾液中长达28周，以便在测试表面形成腐蚀层。还对未腐蚀的凝固汞合金试样进行了测试。所有未腐蚀的凝固汞合金均未显示出细胞毒性产物在表面累积的迹象，而腐蚀后的汞合金则表现出不同程度的细胞毒性。一般来说，不含γ2相汞合金比传统汞合金产生更明显的细胞毒性作用。当在pH值为7的条件下进行腐蚀过程时，各种不含γ2相汞合金表现出不同程度的细胞毒性。不含γ2相汞合金与传统汞合金之间细胞毒性作用的差异以及各种不含γ2相汞合金之间的差异，似乎可能与沉积在汞合金表面的腐蚀产物的保留情况差异有关。
