School of Medical Technology and Information Engineering, Zhejiang Chinese Medical University, Hangzhou 310053, China.
College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.
Sci Total Environ. 2024 May 20;926:172115. doi: 10.1016/j.scitotenv.2024.172115. Epub 2024 Apr 1.
Manure composting in traditional small-scale pig farms leads to the migration and diffusion of antibiotics and antibiotics resistance genes (ARGs) along the chain of transmission to the surrounding environment, increasing the risk of environmental resistance. Understanding the transmission patterns, driving factors, and health risks of ARGs on small-scale pig farms is important for effective control of ARGs transmission. This study was conducted on a small pig farm and its surrounding environment. The cross-media transmission of ARGs and their risks in the farming habitat were investigated using Metagenomic annotation and qPCR quantitative detection. The results indicate that ARGs in farms spread with manure pile-soil-channel sediment-mudflat sediment. Pig farm manure contributed 22.49 % of the mudflat sediment ARGs. Mobile genetic elements mediate the spread of ARGs across different media. Among them, tnpA and IS26 have the highest degree. Transmission of high-risk ARGs sul1 and tetM resulted in a 50 % and 116 % increase in host risk for sediment, respectively. This study provides a basis for farm manure management and control of the ARGs spread.
传统小型养猪场的粪便堆肥导致抗生素沿传播链向周围环境迁移和扩散，并增加了环境耐药性的风险。了解小型养猪场抗生素耐药基因（ARGs）的传播模式、驱动因素和健康风险对于有效控制 ARGs 的传播至关重要。本研究以小型养猪场及其周围环境为对象，采用宏基因组注释和 qPCR 定量检测的方法，研究了养殖栖息地中 ARGs 的跨媒体传播及其风险。结果表明，农场中的 ARGs 随粪堆-土壤-沟渠沉积物-滩涂沉积物传播。养猪场粪肥贡献了滩涂沉积物中 22.49%的 ARGs。移动遗传元件介导了 ARGs 在不同介质之间的传播。其中，tnpA 和 IS26 的传播程度最高。高风险 ARGs sul1 和 tetM 的传播使沉积物中宿主的风险分别增加了 50%和 116%。本研究为养殖场粪肥管理和 ARGs 传播控制提供了依据。
