School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China; Guangdong Province Laborary of Chemistry and Fine Chemical Engineering Jieyang Center, Jieyang, China.
School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou 510006, China.
Int J Biol Macromol. 2024 May;267(Pt 1):131304. doi: 10.1016/j.ijbiomac.2024.131304. Epub 2024 Apr 1.
The study aimed to fabricate β-Lactoglobulin-catechin (β-La-Ca) conjugates as a natural designed antioxidant emulsifier to improve the physicochemical stability of resveratrol emulsion delivery system. Fourier transform infrared (FT-IR) and fluorescence spectroscopy analysis confirmed the formation of conjugates using free radical grafting. The antioxidant ability of emulsion was evaluated by DPPH scavenging activities and ORAC experiments. The emulsion stabilized by β-La-Ca conjugates exhibited strong antioxidant activity with ORAC value of 2541.39 ± 29.58 μmol TE/g, which was significantly higher than that by β-Lactoglobulin alone with 387.96 ± 23.45 μmol TE/g or their mixture with 948.23 ± 32.77 μmol TE/g. During the whole simulated gastrointestinal digestion, emulsion stabilized by β-La-Ca conjugates exhibited excellent oxidative stability that the lipid was mainly digested in the small intestine. This behavior attributed to the greater stability of resveratrol to chemical transformation leading to a higher overall bioavailability in vivo. These results suggested that the β-La-Ca conjugates could be used to fabricate the emulsion-based delivery system to improve the oxidative stability and bioavailability of chemically labile hydrophobic bioactive compounds.
本研究旨在制备β-乳球蛋白-儿茶素（β-La-Ca）缀合物作为一种天然设计的抗氧化乳化剂，以提高白藜芦醇乳液传递系统的物理化学稳定性。傅里叶变换红外（FT-IR）和荧光光谱分析证实了自由基接枝形成了缀合物。通过 DPPH 清除活性和 ORAC 实验评估了乳液的抗氧化能力。由β-La-Ca 缀合物稳定的乳液表现出较强的抗氧化活性，ORAC 值为 2541.39 ± 29.58 μmol TE/g，明显高于单独使用β-乳球蛋白时的 387.96 ± 23.45 μmol TE/g 或其混合物时的 948.23 ± 32.77 μmol TE/g。在整个模拟胃肠道消化过程中，由β-La-Ca 缀合物稳定的乳液表现出优异的氧化稳定性，脂质主要在小肠中被消化。这种行为归因于白藜芦醇对化学转化的更高稳定性，从而导致体内更高的整体生物利用度。这些结果表明，β-La-Ca 缀合物可用于制备基于乳液的传递系统，以提高化学不稳定的疏水性生物活性化合物的氧化稳定性和生物利用度。
