外周血中白细胞介素10基因的甲基化水平与格雷夫斯病的难治性相关。
Methylation levels of the IL10 gene in peripheral blood are related to the intractability of Graves' disease.
作者信息
Kinoshita Riku, Inoue Naoya, Iwatani Yoshinori, Noguchi Yusuke, Hidaka Yoh, Watanabe Mikio
机构信息
Department of Clinical Laboratory and Biomedical Sciences, Division of Health Sciences, Osaka University Graduate School of Medicine, Yamadaoka 1-7 Suita, Osaka 565-0871, Japan.
Department of Clinical Laboratory and Biomedical Sciences, Division of Health Sciences, Osaka University Graduate School of Medicine, Yamadaoka 1-7 Suita, Osaka 565-0871, Japan; Laboratory for Clinical Investigation, Osaka University Hospital, Yamadaoka 2-15, Suita, Osaka 565-0871, Japan.
出版信息
Clin Immunol. 2024 Jun;263:110196. doi: 10.1016/j.clim.2024.110196. Epub 2024 Apr 1.
The prognosis of autoimmune thyroid diseases (AITDs), including Hashimoto's disease (HD) and Graves' disease (GD), is difficult to predict. DNA methylation regulates gene expression of immune mediating factors. Interleukin (IL)-10 is a Th2 cytokine that downregulates inflammatory cytokines produced by Th1 cells. To clarify the role of methylation of the IL10 gene in the prognosis of AITD, we evaluated the methylation levels of two CpG sites in the IL10 promoter using pyrosequencing. The methylation levels of the -185 CpG site of the IL10 gene were related to age and GD intractability in GD patients. Furthermore, the C carrier of the IL10-592 A/C polymorphism was related to low methylation levels of the -185 CpG site. The methylation levels of the IL10-185 CpG site of the IL10 gene were related to the intractability of GD and were lower in individuals with the C allele of the IL10-592 A/C polymorphism.
自身免疫性甲状腺疾病（AITD），包括桥本氏病（HD）和格雷夫斯病（GD），其预后难以预测。DNA甲基化调节免疫介导因子的基因表达。白细胞介素（IL）-10是一种Th2细胞因子，可下调Th1细胞产生的炎性细胞因子。为了阐明IL10基因甲基化在AITD预后中的作用，我们使用焦磷酸测序法评估了IL10启动子中两个CpG位点的甲基化水平。IL10基因-185 CpG位点的甲基化水平与GD患者的年龄和GD难治性有关。此外，IL10-592 A/C多态性的C携带者与-185 CpG位点的低甲基化水平有关。IL10基因IL10-185 CpG位点的甲基化水平与GD的难治性有关，在具有IL10-592 A/C多态性C等位基因的个体中较低。
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