Engineering and finetuning expression of SerC for balanced metabolic flux in vitamin B production.

Vitamin B plays a crucial role in cellular metabolism and stress response, making it an essential component for growth in all known organisms. However, achieving efficient biosynthesis of vitamin B faces the challenge of maintaining a balanced distribution of metabolic flux between growth and production. In this study, our focus is on addressing this challenge through the engineering of phosphoserine aminotransferase (SerC) to resolve its redundancy and promiscuity. The enzyme SerC was semi-designed and screened based on sequences and predicted values, respectively. Mutants and heterologous proteins showing potential were then fine-tuned to optimize the production of vitamin B. The resulting strain enhances the production of vitamin B, indicating that different fluxes are distributed to the biosynthesis pathway of serine and vitamin B. This study presents a promising strategy to address the challenge posed by multifunctional enzymes, with significant implications for enhancing biochemical production through engineering processes.