College of Life Sciences, Northwest University, Xi'an 710069, China P.R.
Anal Chem. 2024 Apr 16;96(15):5741-5745. doi: 10.1021/acs.analchem.4c00445. Epub 2024 Apr 4.
Fucosylation is an important structural feature of glycans and plays an essential role in the regulation of glycoprotein functions. Fucosylation can be classified into core- (CF) and antenna-fucosylation (AF, also known as (sialyl-) Lewis) based on the location on -glycans, and they perform distinct biological functions. In this study, core- and antenna-fucosylated -glycans on human serum glycoproteins that hold great clinical application values were systematically characterized at the site-specific level using StrucGP combined with the recently developed fucosylation assignment method. The results showed that fucosylation was widely distributed on serum glycoproteins, with 50% of fucosylated glycopeptides modified by AF -glycans, 37% by CF -glycans, and 13% by dual-fucosylated -glycans. Interestingly, CF and AF -glycans preferred to modify different groups of serum glycoproteins with different tissue origins and were involved in distinctive biological processes. Specifically, AF -glycoproteins are mainly from the liver and participated in complement activation, blood coagulation, and endopeptidase activities, while CF -glycoproteins originate from diverse tissues and are mainly involved in cell adhesion and signaling transduction. These data further enhanced our understanding of fucosylation on circulation glycoproteins.
糖基化是聚糖的一个重要结构特征，在糖蛋白功能的调节中起着至关重要的作用。根据 -聚糖上的位置，糖基化可分为核心（CF）和天线糖基化（AF，也称为（唾液酸）-Lewis），它们具有不同的生物学功能。在这项研究中，使用 StrucGP 结合最近开发的糖基化分配方法，在特定位置上系统地描述了具有重要临床应用价值的人血清糖蛋白上的核心和天线糖基化 -聚糖。结果表明，糖基化广泛分布于血清糖蛋白上，其中 50%的糖肽被 AF -聚糖修饰，37%被 CF -聚糖修饰，13%被双糖基化 -聚糖修饰。有趣的是，CF 和 AF -聚糖更喜欢修饰具有不同组织来源的不同组的血清糖蛋白，并参与不同的生物学过程。具体而言，AF -聚糖蛋白主要来自肝脏，并参与补体激活、血液凝固和内肽酶活性，而 CF -聚糖蛋白来源于多种组织，主要参与细胞黏附和信号转导。这些数据进一步增强了我们对循环糖蛋白上糖基化的理解。
