Koeffler H P, Yelton L, Prokocimer M, Hirji K
Leuk Res. 1985;9(1):73-81. doi: 10.1016/0145-2126(85)90022-0.
The human promyelocytic leukemia cell line known as HL-60 can be triggered to mature to functional granulocytes and/or macrophages after exposure to a variety of compounds. The findings have generated enthusiasm for possible therapy of leukemia using compounds that induce leukemic cell differentiation. We investigated whether five compounds known to trigger HL-60 differentiation to granulocytes could trigger the maturation of blast cells from 12 patients with myelogenous leukemia. Maturation was judged by morphology, superoxide production, phagocytosis, expression of Fc receptors, and development of alpha-napthyl acetate esterase activity. The blast cells from most patients showed little morphological, histological or functional maturation after exposure to the various compounds as compared to the blast cells cultured without the compounds. Actinomycin was able to induce significant maturation of leukemic cells of some patients when maturation was analyzed by several statistical methods. Our study suggests that many compounds which trigger differentiation of promyelocytic leukemia cells may not trigger differentiation of less mature myeloid leukemic cells.
人早幼粒细胞白血病细胞系HL - 60在接触多种化合物后可被诱导成熟为功能性粒细胞和/或巨噬细胞。这些发现激发了人们对于使用诱导白血病细胞分化的化合物治疗白血病的热情。我们研究了五种已知可触发HL - 60向粒细胞分化的化合物是否能促使12例骨髓性白血病患者的原始细胞成熟。通过形态学、超氧化物生成、吞噬作用、Fc受体表达以及α - 萘乙酸酯酶活性的发展来判断成熟情况。与未接触化合物培养的原始细胞相比，大多数患者的原始细胞在接触各种化合物后，在形态学、组织学或功能上几乎没有成熟迹象。当通过几种统计方法分析成熟情况时，放线菌素能够诱导部分患者的白血病细胞显著成熟。我们的研究表明，许多能触发早幼粒细胞白血病细胞分化的化合物可能无法触发较不成熟的髓系白血病细胞分化。
