Collins S J, Ruscetti F W, Gallagher R E, Gallo R C
Proc Natl Acad Sci U S A. 1978 May;75(5):2458-62. doi: 10.1073/pnas.75.5.2458.
A human leukemic cell line (designated HL-60) has recently been established from the peripheral blood leukocytes of a patient with acute promyelocytic leukemia. This cell line displays distinct morphological and histochemical commitment towards myeloid differentiation. The cultured cells are predominantly promyelocytes, but the addition of dimethyl sulfoxide to the culture induces them to differentiate into myelocytes, metamyelocytes, and banded and segmented neutrophils. All 150 clones developed from the HL-60 culture show similar morphological differentiation in the presence of dimethyl sulfoxide. Unlike the morphologically immature promyelocytes, the dimethyl sulfoxide-induced mature cells exhibit functional maturity as exemplified by phagocytic activity. A number of other compounds previously shown to induce erythroid differentiation of mouse erythroleukemia (Friend) cells can induce analogous maturation of the myeloid HL-60 cells. The marked similarity in behavior of HL-60 cells and Friend cells in the presence of these inducing agents suggests that similar molecular mechanisms are involved in the induction of differentiation of these human myeloid and murine erythroid leukemic cells.
一种人类白血病细胞系（命名为HL - 60）最近从一名急性早幼粒细胞白血病患者的外周血白细胞中建立。该细胞系对髓系分化表现出明显的形态学和组织化学特征。培养的细胞主要是早幼粒细胞，但向培养物中添加二甲基亚砜可诱导它们分化为中幼粒细胞、晚幼粒细胞以及带状和分叶核中性粒细胞。从HL - 60培养物中产生的所有150个克隆在二甲基亚砜存在下均表现出相似的形态学分化。与形态上未成熟的早幼粒细胞不同，二甲基亚砜诱导的成熟细胞表现出功能成熟，如吞噬活性所示。先前显示可诱导小鼠红白血病（Friend）细胞红系分化的许多其他化合物，也可诱导髓系HL - 60细胞发生类似的成熟。在这些诱导剂存在下，HL - 60细胞和Friend细胞行为的显著相似性表明，在诱导这些人类髓系和小鼠红系白血病细胞分化过程中涉及相似的分子机制。
