Oketch Elijah Ogola, Yu Myunghwan, Hong Jun Seon, Chaturanga Nuwan Chamara, Seo Eunsoo, Lee Hans, Hermes Rafael Gustavo, Smeets Natasja, Taechavasonyoo Apichaya, Kirwan Susanne, Rodriguez-Sanchez Raquel, Heo Jung Min
Department of Animal Science and Biotechnology, Chungnam National University, Daejeon 34134, Korea.
Kemin Animal Nutrition and Health, Asia Pacific, 12 Senoko Drive, Singapore 758200.
Anim Biosci. 2024 Aug;37(8):1418-1427. doi: 10.5713/ab.23.0495. Epub 2024 Apr 1.
This study aimed to investigate the efficacy of Bacillus-based probiotics supplemented at two different levels to modulate the productive performance, egg quality, tibia traits, and specific cecal bacteria counts of Hy-Line Brown layers from 25 to 37 weeks of age.
A total of 216 twenty-five-week-old hens were randomly distributed into 3 experimental diets with 12 replicates of 6 birds per cage. Diets included basal diet supplemented with 0 (CON), 3×108 (PRO1), or 3×109 (PRO2) colony-forming unit (CFU) of the test probiotic containing Bacillus subtilis PB6, Bacillus subtilis FXA, and Bacillus licheniformis G3 per kilogram of feed.
Improved egg weights and mass at 29 weeks; and feed intake at 31 weeks (p<0.10) were noticed with the probiotic-supplemented PRO1 and PRO2 diets. Considering egg quality, the shell thickness, Haugh units, and yolk color were improved; but yolk cholesterol was lowered (p<0.05) with PRO1 and PRO2 diets at 29 weeks. At both 33 and 37 weeks, the egg-breaking strength, shell color and thickness, albumen height, Haugh units, and yolk color were improved; but yolk cholesterol was similarly lowered (p<0.05) with the PRO1 and PRO2 diets. Improved tibia Ca, ash, weights, and density; and raised cecal counts of Bifidobacteria and Lactobacilli (p<0.05) were noticed with PRO1 and PRO2 diets. Improved tibia P but reduced Clostridia counts (p<0.10) were also observed with the PRO1 and PRO2 diets.
Probiotic supplementation of Bacillus subtilis PB6, Bacillus subtilis FXA, and Bacillus licheniformis G3 at 3×108 CFU/kg of feed is adequate to significantly improve egg quality, lower yolk cholesterol, enhance several tibia traits, and raise the populations of beneficial cecal bacteria. Modest improvements in several productive parameters and tibia P but reduced Clostridia were also observed; and could warrant further investigation of probiotic effects beyond the current test period.
本研究旨在调查添加两种不同水平的芽孢杆菌属益生菌对25至37周龄海兰褐蛋鸡生产性能、蛋品质、胫骨性状和盲肠特定细菌数量的调节作用。
将总共216只25周龄母鸡随机分为3种实验日粮组，每组12个重复，每个笼子6只鸡。日粮包括基础日粮，分别添加每千克饲料0（对照组）、3×10⁸（PRO1）或3×10⁹（PRO2）菌落形成单位（CFU）的含枯草芽孢杆菌PB6、枯草芽孢杆菌FXA和地衣芽孢杆菌G3的试验益生菌。
添加益生菌的PRO1和PRO2日粮组在29周龄时蛋重和蛋重有所提高；在31周龄时采食量增加（p<0.10）。在蛋品质方面，29周龄时，PRO1和PRO2日粮组的蛋壳厚度、哈夫单位和蛋黄颜色得到改善；但蛋黄胆固醇降低（p<0.05）。在33周龄和37周龄时，PRO1和PRO2日粮组的蛋壳强度、蛋壳颜色和厚度、蛋白高度、哈夫单位和蛋黄颜色均得到改善；但蛋黄胆固醇同样降低（p<0.05）。PRO1和PRO2日粮组的胫骨钙、灰分、重量和密度得到改善；盲肠双歧杆菌和乳酸杆菌数量增加（p<0.05）。PRO1和PRO2日粮组还观察到胫骨磷增加但梭菌数量减少（p<0.10）。
每千克饲料添加3×10⁸CFU的枯草芽孢杆菌PB6、枯草芽孢杆菌FXA和地衣芽孢杆菌G3的益生菌足以显著改善蛋品质、降低蛋黄胆固醇、增强多项胫骨性状并增加有益盲肠细菌数量。还观察到几个生产参数和胫骨磷有适度改善，但梭菌数量减少；这可能值得在当前试验期之后进一步研究益生菌的作用。
