Pfeffer L M, Kwok B C, Landsberger F R, Tamm I
Proc Natl Acad Sci U S A. 1985 Apr;82(8):2417-21. doi: 10.1073/pnas.82.8.2417.
Treatment of human HeLa-S3 cells (an epidermoid carcinoma line) with human beta-interferon (640 units/ml) selectively alters lipid metabolism by increasing cholesterol synthesis per mg of cell protein as measured by 1-hr pulse-labeling of cells with [3H]acetate. Cholesterol synthesis in interferon-treated cells is increased approximately equal to 60% at 24 hr after the beginning of treatment and approximately equal to 450% at 48 hr. Continuous labeling of interferon-treated cells with [14C]acetate shows increased accumulation of label in cholesterol when normalized per mg of cell protein, as well as an increase in the specific activity of cholesterol in the treated cells. In contrast, interferon treatment decreases the accumulation of [14C]acetate into cholesterol esters. The [14C]acetate labeling of sphingomyelin, phosphatidylethanolamine, and triglycerides shows no change compared to untreated controls. The labeling of phosphatidylcholine was moderately increased in treated cells. The interferon-induced changes in lipid metabolism are a part of a coordinated response of cells to interferon treatment, characterized by reduced cell proliferation and cell motility and an increase in cell size and mass. The increased cholesterol synthesis is consistent with a model in which beta-interferon treatment of HeLa cells inhibits the endocytosis of cholesterol-containing low density lipoprotein, which results in an increase in cholesterol synthesis.
用人β-干扰素（640单位/毫升）处理人HeLa-S3细胞（一种表皮样癌细胞系），通过用[3H]乙酸对细胞进行1小时脉冲标记来测量，可选择性地改变脂质代谢，即增加每毫克细胞蛋白的胆固醇合成。在开始处理后24小时，经干扰素处理的细胞中的胆固醇合成增加约60%，在48小时时增加约450%。用[14C]乙酸对经干扰素处理的细胞进行连续标记显示，当以每毫克细胞蛋白进行标准化时，胆固醇中标记的积累增加，并且处理细胞中胆固醇的比活性也增加。相比之下，干扰素处理会减少[14C]乙酸向胆固醇酯中的积累。与未处理的对照相比，鞘磷脂、磷脂酰乙醇胺和甘油三酯的[14C]乙酸标记没有变化。处理细胞中磷脂酰胆碱的标记适度增加。干扰素诱导的脂质代谢变化是细胞对干扰素处理的协调反应的一部分，其特征是细胞增殖和细胞运动性降低，细胞大小和质量增加。胆固醇合成增加与一种模型一致，在该模型中，β-干扰素处理HeLa细胞会抑制含胆固醇的低密度脂蛋白的内吞作用，从而导致胆固醇合成增加。
