Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt.
Department of Environmental Studies, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt.
Microb Cell Fact. 2024 Apr 4;23(1):102. doi: 10.1186/s12934-024-02374-5.
Poultry feather waste has a potential for bioenergy production because of its high protein content. This research explored the use of chicken feather hydrolysate for methane and hydrogen production via anaerobic digestion and bioelectrochemical systems, respectively. Solid state fermentation of chicken waste was conducted using a recombinant strain of Bacillus subtilis DB100 (p5.2).
In the anaerobic digestion, feather hydrolysate produced maximally 0.67 Nm CH/kg feathers and 0.85 mmol H/day.L concomitant to COD removal of 86% and 93%, respectively. The bioelectrochemical systems used were microbial fuel and electrolysis cells. In the first using a microbial fuel cell, feather hydrolysate produced electricity with a maximum cell potential of 375 mV and a current of 0.52 mA. In the microbial electrolysis cell, the hydrolysate enhanced the hydrogen production rate to 7.5 mmol/day.L, with a current density of 11.5 A/m and a power density of 9.26 W/m.
The data indicated that the sustainable utilization of keratin hydrolysate to produce electricity and biohydrogen via bioelectrical chemical systems is feasible. Keratin hydrolysate can produce electricity and biofuels through an integrated aerobic-anaerobic fermentation system.
家禽羽毛废弃物由于其高蛋白含量，具有生物能源生产的潜力。本研究分别探索了利用鸡羽毛水解物通过厌氧消化和生物电化学系统生产甲烷和氢气。使用枯草芽孢杆菌 DB100（p5.2）的重组菌株对鸡废物进行固态发酵。
在厌氧消化中，羽毛水解物分别产生了最大的 0.67 Nm CH/kg 羽毛和 0.85 mmol H/天·L，同时 COD 去除率分别为 86%和 93%。所使用的生物电化学系统是微生物燃料电池和电解槽。在第一个使用微生物燃料电池的系统中，羽毛水解物产生了最大 375 mV 的电池电势和 0.52 mA 的电流。在微生物电解槽中，水解物将氢气的产率提高到 7.5 mmol/天·L，电流密度为 11.5 A/m，功率密度为 9.26 W/m。
数据表明，通过生物电化学系统利用角蛋白水解物可持续地生产电力和生物氢气是可行的。角蛋白水解物可以通过集成的好氧-厌氧发酵系统产生电力和生物燃料。
