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基于蛋白质ORF2和ORF3的戊型肝炎病毒多表位疫苗设计

Multi-epitope vaccine design for hepatitis E virus based on protein ORF2 and ORF3.

作者信息

Lu Qiong, Wu Hao, Meng Jing, Wang Jiangyuan, Wu Jiajing, Liu Shuo, Tong Jincheng, Nie Jianhui, Huang Weijin

机构信息

Division of HIV/AIDS and Sex-transmitted Virus Vaccines, Institute for Biological Product Control, National Institutes for Food and Drug Control and WHO Collaborating Center for Standardization and Evaluation of Biologicals, Beijing, China.

Wuhan Institute of Biological Products Co., Ltd., Wuhan, China.

出版信息

Front Microbiol. 2024 Mar 21;15:1372069. doi: 10.3389/fmicb.2024.1372069. eCollection 2024.

DOI:10.3389/fmicb.2024.1372069
PMID:38577684
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10991829/
Abstract

INTRODUCTION

Hepatitis E virus (HEV), with heightened virulence in immunocompromised individuals and pregnant women, is a pervasive threat in developing countries. A globaly available vaccine against HEV is currently lacking.

METHODS

We designed a multi-epitope vaccine based on protein ORF2 and ORF3 of HEV using immunoinformatics.

RESULTS

The vaccine comprised 23 nontoxic, nonallergenic, soluble peptides. The stability of the docked peptide vaccine-TLR3 complex was validated by molecular dynamic simulations. The induction of effective cellular and humoral immune responses by the multi-peptide vaccine was verified by simulated immunization.

DISCUSSION

These findings provide a foundation for future HEV vaccine studies.

摘要

引言

戊型肝炎病毒（HEV）在免疫功能低下的个体和孕妇中具有更高的毒力，是发展中国家普遍存在的威胁。目前全球缺乏针对HEV的可用疫苗。

方法

我们利用免疫信息学设计了一种基于HEV蛋白ORF2和ORF3的多表位疫苗。

结果

该疫苗由23种无毒、无过敏、可溶性肽组成。通过分子动力学模拟验证了对接肽疫苗-TLR3复合物的稳定性。通过模拟免疫验证了多肽疫苗诱导有效的细胞免疫和体液免疫反应。

讨论

这些发现为未来的HEV疫苗研究奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0a3/10991829/25f0dd79ae5a/fmicb-15-1372069-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0a3/10991829/2d62a8336c61/fmicb-15-1372069-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0a3/10991829/fd2eaf81514c/fmicb-15-1372069-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0a3/10991829/48d732376137/fmicb-15-1372069-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0a3/10991829/4cf373f17ec3/fmicb-15-1372069-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0a3/10991829/25f0dd79ae5a/fmicb-15-1372069-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0a3/10991829/2d62a8336c61/fmicb-15-1372069-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0a3/10991829/fd2eaf81514c/fmicb-15-1372069-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0a3/10991829/48d732376137/fmicb-15-1372069-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0a3/10991829/4cf373f17ec3/fmicb-15-1372069-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0a3/10991829/25f0dd79ae5a/fmicb-15-1372069-g005.jpg

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