School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 311402, China.
Fuyang Research Institute, Zhejiang Chinese Medical University, Hangzhou 310053, China.
J Agric Food Chem. 2024 Apr 17;72(15):8632-8649. doi: 10.1021/acs.jafc.4c01351. Epub 2024 Apr 5.
Our previous studies found that Sea Buckthorn polyphenols (SBP) extract inhibits fatty acid synthase (FAS) . Thus, we continued to explore possible effects and underlying mechanisms of SBP on complicated metabolic disorders in long-term high-fat-diet (HFD)-fed mice. To reveal that, an integrated approach was developed in this study. Targeted quantitative lipidomics with a total of 904 unique lipids mapping contributes to profiling the comprehensive features of disarranged hepatic lipid homeostasis and discovering a set of newfound lipid-based biomarkers to predict the occurrence and indicate the progression of metabolic disorders beyond current indicators. On the other hand, technologies of intermolecular interactions characterization, especially surface plasmon resonance (SPR) assay, contribute to recognizing targeted bioactive constituents present in SBP. Our findings highlight hepatic lipid homeostasis maintenance and constituent-FAS enzyme interactions, to provide new insights that SBP as a functional food alleviates HFD-induced metabolic disorders in mice reprograming hepatic lipid homeostasis caused by targeting FAS, owing to four polyphenols directly interacting with FAS and cinaroside binding to FAS with good affinity.
我们之前的研究发现，沙棘多酚（SBP）提取物抑制脂肪酸合酶（FAS）。因此，我们继续探索 SBP 对长期高脂肪饮食（HFD）喂养小鼠复杂代谢紊乱的可能影响和潜在机制。为了揭示这一点，本研究采用了一种综合方法。靶向定量脂质组学共鉴定了 904 种独特的脂质，有助于分析肝脏脂质代谢紊乱的综合特征，并发现了一组新的基于脂质的生物标志物，以预测代谢紊乱的发生，并指示其超出当前指标的进展。另一方面，分子间相互作用特征的技术，特别是表面等离子体共振（SPR）分析，有助于识别 SBP 中存在的靶向生物活性成分。我们的研究结果强调了肝脏脂质代谢平衡的维持和成分-FAS 酶的相互作用，为 SBP 作为一种功能性食品缓解 HFD 诱导的小鼠代谢紊乱提供了新的见解，其通过靶向 FAS 重新编程肝脏脂质代谢平衡，这归因于四种多酚直接与 FAS 相互作用，而迷迭香苷与 FAS 具有良好的亲和力结合。
