Saravis C A, O'Brien M, Zamcheck N
J Immunol Methods. 1979;29(1):97-100. doi: 10.1016/0022-1759(79)90131-5.
We are isoelectric focusing small amounts of tissues placed directly on electroendosmosis-free agarose gels instead of using the saline extracts of tissue homogenates. More numerous proteins are extracted during isoelectric focusing of the solid tissues than present in the same tissues.
我们正在对直接放置在无电渗的琼脂糖凝胶上的少量组织进行等电聚焦,而不是使用组织匀浆的盐提取物。在固体组织的等电聚焦过程中提取的蛋白质比相同组织中存在的蛋白质更多。