琼脂糖中的制备性等电聚焦
Preparative isoelectric focusing in agarose.
作者信息
Ebers G C, Rice G P, Armstrong H
出版信息
J Immunol Methods. 1980;37(3-4):315-23. doi: 10.1016/0022-1759(80)90317-8.
PMID:7451998
Abstract
A method is described for preparative isoelectric focusing in agarose using low electroendosmotic agarose. Resolution comparable to that seen on analytical polyacrylamide gels is attainable as demonstrated by the isolation of bands with identical idiotypes from the serum of a patient with a monoclonal gammopathy.
摘要
本文描述了一种使用低电渗琼脂糖在琼脂糖中进行制备性等电聚焦的方法。从一名单克隆丙种球蛋白病患者的血清中分离出具有相同独特型的条带，这表明该方法可获得与分析型聚丙烯酰胺凝胶相当的分辨率。
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