Department of Biological Chemistry, University of Michigan, Ann Arbor, MI, USA; Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA; Center for RNA Biomedicine, University of Michigan, Ann Arbor, MI, USA.
Structure. 2024 Apr 4;32(4):377-379. doi: 10.1016/j.str.2024.03.001.
Eiler et al. used cryo-electron microscopy to determine a 2.49 Å resolution structure of Giardia lamblia 80S ribosome bound to tRNA, mRNA, and the anti-protozoal drug emetine. The structure reveals some critical aspects of translation in G. lamblia, including the lack of ribosomal protein RACK1, and how emetine blocks translation by interacting with both the ribosome and mRNA.
Eiler 等人使用冷冻电子显微镜确定了 2.49Å 分辨率的蓝氏贾第鞭毛虫 80S 核糖体与 tRNA、mRNA 和抗原生动物药物依米丁结合的结构。该结构揭示了蓝氏贾第鞭毛虫翻译的一些关键方面，包括核糖体蛋白 RACK1 的缺失，以及依米丁如何通过与核糖体和 mRNA 相互作用来阻止翻译。
